Xanthomonas campestris pv. pelargonii (Xcp) - DAS ELISA                           Home    Products    Testing Services   Supplies

Catalog #:  B109
Source Antibody:  Rabbit polyclonal antibodies for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This ELISA reacted with all Xcp isolates tested.  Reaction of this test is strong.  Optical Density at 405nm  is in a range of 1.200 - 3.000 depending on the bacterial titers in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The bacterium can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:   This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis, C. m. subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, Erwinia chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. citri,  X. c. pv. dieffenbachiae, X. c. pv. phaseoli, and X. c. pv. vesicatoria.  This ELISA shows cross-reaction with Xanthomonas campestris pv. armoraciae and some cross-reaction (16%) with X. c. pv. campestris  
There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect Xcp in petioles, stems, leaves and roots of infected host plants as well as in pure bacterial cultures.

Products: 

Catalog Number

Description

Test Wells

Price
  $US

Ship Weight
(Estimated)

B109-R1

ELISA Reagents, Alkaline Phosphatase

500

220

2 lb (1 kg)

B109-R2

ELISA Reagents, Alkaline Phosphatase

1000

385

3 lb (1.5 kg)

B109-R3

ELISA Reagents, Alkaline Phosphatase

5000

1515

12 lb (6 kg)
N109-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
B109-K1 ELISA Kit, Alkaline Phosphatase

500

330

2 lb (1 kg)
B109-K2 ELISA Kit, Alkaline Phosphatase

1000

520

3 lb (1.5 kg)

B109-C1

Coating Antibody

500

110

2 lb (1 kg)

B109-C2

Coating Antibody

1000

195

2 lb (1 kg)

B109-C3

Coating Antibody

5000

690

2 lb (1 kg)

B109-D1

Detecting conjugate, Alkaline Phosphatase

500

135

2 lb (1 kg)

B109-D2

Detecting conjugate, Alkaline Phosphatase

1000

215

2 lb (1 kg)

B109-D3

Detecting conjugate, Alkaline Phosphatase

5000

860

2 lb (1 kg)
BS109-LF1  Lateral flow device (Immunostrip)

10

65

2 lb (1 kg)
BS109-LF2  Lateral flow device (Immunostrip)

25

90

3 lb (1.5 kg)
BS109-LF3  Lateral flow device (Immunostrip)

100

290

5 lb (2 kg)

B109-P1

Positive control

18

15

1 lb (0.5 kg)

B109-N1

Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500    90 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000     120 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000     350 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Bacterium

Name:  Xanthomonas campestris pv. pelargonii 
Acronym:  Xcp
Synonyms: 
Group/Genus:  Xanthomonas

Host plants and Diseases:   Bacterial blight of geranium
Symptoms:  Leaf blight, plant wilting.

Bacterial Transmission:   Vegetative propagation.
Bacterial Infection:  Systemic infection in geranium plant.

Reference:


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