Xanthomonas campestris pv. campestris (Xcc) - DAS ELISA                          Home    Products    Testing Services   Supplies

Catalog #:  B107
Source Antibody:  Rabbit polyclonal antibodies for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This ELISA reacted with all Xcc isolates tested.  Reaction of the ELISA is strong.  Optical Density at 405_nm  is in a range of 1.300 - 2.800 depending on the bacterial titers in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The bacterium can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis, C. m. subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, Erwinia chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. citri, X. c. pv. diffenbachiae,  X. c. pv. pelargonii, X. c. pv. phaseoli, and X. c. pv. vesicatoria.  This test shows some cross-reaction with Xanthomonas campestris pv. armoraciae (30-40%).  
There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect Xcc in infected host plants as well as in pure bacterial cultures.

Products: 

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To know more about the products:  Product Items
To learn how to perform the test: Instructions for DAS ELISA 
To be familiar with other components: Buffer Sets; Controls

Information About the Bacterium

Name:  Xanthomonas campestris pv. campestris 
Acronym:  Xcc
Synonyms: 
Group/Genus:  Xanthomonas

Host plants and Diseases:  Black rot of crucifers
Symptoms:  Large, yellow to dark brown lesions formed along leaf margins. Also, plant dwarfing or wilting. 

Bacterial Transmission:  Seeds internally infected or externally infected.  Diseased plant residues on or in the ground.
Bacterial Infection:  The pathogen invades the vascular tissue of leaves, stalks, stumps and roots.  A very small portion of infected seeds or primary infection has the potential to induce epidemics during later seasons.  The secondary infection is caused by rain splash or irrigation water.

Reference:

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