Wheat Streak Mosaic Virus (WSMV) - DAS ELISA                                Home    Products    Testing Services   Supplies

Catalog #:  V067
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all WSMV isolates tested.  Reaction of the ELISA is moderately strong.  Optical Density at 405nm  is in a range of 0.600 - 1.600 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderately high.  The virus can be consistently detected in infected plant leaf tissues diluted  at 1:270 -  1:810.

Specificity:  There is no obvious cross reaction with healthy plant tissues.  Background is very low on the negative control wells.

Application:  The test can be used to detect WSMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $US Ship Weight
(Estimated)

V067-R1

ELISA Reagents, Alkaline Phosphatase

500

210

2 lb (1 kg)

V067-R2

ELISA Reagents, Alkaline Phosphatase

1000

370

3 lb (1.5 kg)

V067-R3

ELISA Reagents, Alkaline Phosphatase

5000

1450

12 lb (6 kg)
V067-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V067-K1 ELISA Kit, Alkaline Phosphatase

500

325

2 lb (1 kg)
V067-K2 ELISA Kit, Alkaline Phosphatase

1000

505

3 lb (1.5 kg)

V067-C1

Coating Antibody

500

105

2 lb (1 kg)

V067-C2

Coating Antibody

1000

185

2 lb (1 kg)

V067-C3

Coating Antibody

5000

660

2 lb (1 kg)

V067-D1

Detecting conjugate, Alkaline Phosphatase

500

130

2 lb (1 kg)

V067-D2

Detecting conjugate, Alkaline Phosphatase

1000

205

2 lb (1 kg)

V067-D3

Detecting conjugate, Alkaline Phosphatase

5000

820

2 lb (1 kg)

V067-P1

Positive control

18

15

1 lb (0.5 kg)

V067-N1

Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500    90 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000     120 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000     350 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA Reagents
To learn how to perform the Pathogen ID test: Instructions for DAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Wheat Streak Mosaic Virus
Acronym:  WSMV
Synonyms:  Marmor virgatum, wheat virus 6, wheat virus 7.
Group/Genus:  Rymoviruses

Vector:   Mites
Transmission:  Seed
Main host plants:  Wheat, barley, millet, maize, oats, rye.

Viral Particle:  RNA virus with filamentous particles, 700 nm long and 15 nm in diameter.
Virus Infection:  Wheat streak mosaic virus (WSMV) infection causes systemic mosaic symptoms under both warm and cool temperatures.  When wheat is infected in autumn, plants show severe stunting, varying degrees of necrosis and death of tillers, and reduced seed set and seed weight.  WSMV is transmitted by the wheat-curl mite, which acquires the virus from the plant only by nymphs, and transmits the virus by both nymphs and adults of the mite.  The virus does not pass from the adult mite to its progeny through egg.  WSMV is transmitted by seed in very low levels.

 WSMV  is found in Canada, Jordan, Romania, Russia,  the USA and Yugoslavia.  WSMV is severe in  the south-central part of the United States, where a host continuum exists for both mite and virus between spring and autumn-sown crops.

Reference:
1.
  CMI/AAB Descriptions of Plant Viruses, No. 48.


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