Wheat Streak Mosaic Virus (WSMV) - TAS ELISA                             Home    Products    Testing Services   Supplies

Catalog #:  V067
Source Antibody:  A rabbit polyclonal antibody for capture and a monoclonal antibody for detection.
Test Format:  TAS ELISA                                   

Reactivity:  This test system reacted with all WSMV isolates tested.  Reaction of the ELISA is relatively strong.  Optical Density at 405nm  is in a range of 0.700 - 1.900 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant leaf tissues diluted  at 1:810.

Specificity:  There is no obvious cross reaction with healthy plant tissues.  Background is very low on the negative control wells.

Application:  The test can be used to detect WSMV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V067-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V067-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V067-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V067-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V067-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V067-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V067-C1

Coating Antibody

500

115

2 lb (1 kg)

V067-C2

Coating Antibody

1000

205

2 lb (1 kg)

V067-C3

Coating Antibody

5000

725

2 lb (1 kg)

V067-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V067-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V067-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)

V067-P1

Positive control

18

20

1 lb (0.5 kg)

V067-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Wheat Streak Mosaic Virus
Acronym:  WSMV
Synonyms:  Marmor virgatum, wheat virus 6, wheat virus 7.
Group/Genus:  Rymoviruses

Vector:   Mites
Transmission:  Seed
Main host plants:  Wheat, barley, millet, maize, oats, rye.

Viral Particle:  RNA virus with filamentous particles, 700 nm long and 15 nm in diameter.
Virus Infection:  Wheat streak mosaic virus (WSMV) infection causes systemic mosaic symptoms under both warm and cool temperatures.  When wheat is infected in autumn, plants show severe stunting, varying degrees of necrosis and death of tillers, and reduced seed set and seed weight.  WSMV is transmitted by the wheat-curl mite, which acquires the virus from the plant only by nymphs, and transmits the virus by both nymphs and adults of the mite.  The virus does not pass from the adult mite to its progeny through egg.  WSMV is transmitted by seed in very low levels.

 Geographical distribution: WSMV  is found in Canada, Jordan, Romania, Russia,  the USA and Yugoslavia.  WSMV is severe in  the south-central part of the United States, where a host continuum exists for both mite and virus between spring and autumn-sown crops.

Reference:
1.
  CMI/AAB Descriptions of Plant Viruses, No. 48.


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