Watermelon  Mosaic Virus - 2 (WMV-2) - DAS ELISA                          Home    Products    Testing Services   Supplies

Catalog #:  V065
Source Antibody:  A rabbit polyclonal antibody for capture and a monoclonal antibody for detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all MWV-2 isolates tested.  Reaction of the ELISA is very strong.  Optical Density at 405nm  is in a range of 1.300 - > 3.200 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant leaf tissues diluted  at 1:810 - 1:2430. This test can be used to detect WMV-2 in seeds.

Specificity:  There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect WMV-2 in infected host plant tissues and seeds.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V065-R1

ELISA Reagents, Alkaline Phosphatase

500

220

2 lb (1 kg)

V065-R2

ELISA Reagents, Alkaline Phosphatase

1000

385

3 lb (1.5 kg)

V065-R3

ELISA Reagents, Alkaline Phosphatase

5000

1515

12 lb (6 kg)
V065-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V065-K1 ELISA Kit, Alkaline Phosphatase

500

340

2 lb (1 kg)
V065-K2 ELISA Kit, Alkaline Phosphatase

1000

530

3 lb (1.5 kg)

V065-C1

Coating Antibody

500

110

2 lb (1 kg)

V065-C2

Coating Antibody

1000

195

2 lb (1 kg)

V065-C3

Coating Antibody

5000

690

2 lb (1 kg)

V065-D1

Detecting conjugate, Alkaline Phosphatase

500

135

2 lb (1 kg)

V065-D2

Detecting conjugate, Alkaline Phosphatase

1000

215

2 lb (1 kg)

V065-D3

Detecting conjugate, Alkaline Phosphatase

5000

860

2 lb (1 kg)

V065-P1

Positive control

18

18

1 lb (0.5 kg)

V065-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000  130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000  400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELIAS
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Watermelon Mosaic Virus - 2
Acronym:  WMV-2
Synonyms:  General watermelon mosaic virus
Group/Genus:  Potyviruses

Vector:   Aphids, in non-persistent manner.
Transmission:  Mechanical
Main host plants:  Wide host range

Viral Particle:  Filamentous particles, containing RNA genome.
Virus Infection:  Watermelon mosaic virus - 2 (WMV-2) infects many different plants, including cucurbits, leguminous, and various ornamentals.   WMV-s induces mosaic and mottle symptoms on cantaloupe, cucumber, pea, pumpkin, squash, and watermelon.

Reference:
1.
  CMI/AAB Descriptions of Plant Viruses, No. 63, 293.
2.  Thomas A. Zitter, Donald L. Hopkins and Claude E. Thomas, 1996.  Compendium of Cucurbit Diseases,   APS Press.


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