Pseudomonas savastanoi pv. phaseolicola (Psph) - DAS ELISA               Home    Products    Testing Services   Supplies

Catalog #:  B106
Source Antibody:  Rabbit polyclonal antibodies for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This ELISA reacted with all Psph isolates tested.  Reaction of the ELISA is very strong.  Optical Density at 405nm  is in arrange of 1.400 - 2.900 depending on the bacterial titers in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The bacterium can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. insidiosus and C. m. subsp. nebraskensis, C. m. subsp. michiganensis, C. m. subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, Erwinia chrysanthemi, E. stewartii, Pseudomonas syringae pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c. pv. diffenbachiae,  X. c. pv. pelargonii, X. c. pv. phaseoli, and X. c. pv. vesicatoria.  There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect Psph in infected host plants as well as in pure bacterial cultures.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

B106-R1

ELISA Reagents, Alkaline Phosphatase

500

220

2 lb (1 kg)

B106-R2

ELISA Reagents, Alkaline Phosphatase

1000

385

3 lb (1.5 kg)

B106-R3

ELISA Reagents, Alkaline Phosphatase

5000

1515

12 lb (6 kg)
B106-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
B106-K1 ELISA Kit, Alkaline Phosphatase

500

340

2 lb (1 kg)
B106-K2 ELISA Kit, Alkaline Phosphatase

1000

530

3 lb (1.5 kg)

B106-C1

Coating Antibody

500

110

2 lb (1 kg)

B106-C2

Coating Antibody

1000

195

2 lb (1 kg)

B106-C3

Coating Antibody

5000

690

2 lb (1 kg)

B106-D1

Detecting conjugate, Alkaline Phosphatase

500

135

2 lb (1 kg)

B106-D2

Detecting conjugate, Alkaline Phosphatase

1000

215

2 lb (1 kg)

B106-D3

Detecting conjugate, Alkaline Phosphatase

5000

860

2 lb (1 kg)

B106-P1

Positive control

18

18

1 lb (0.5 kg)

B106-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Bacterium

Name:  Pseudomonas syringae pv. phaseolicola 
Acronym:  Psph
Synonyms: 
Group/Genus:  Pseudomonas

Host plants and Diseases:  Halo blight of beans
Symptoms:  Yellow or chlorotic halo form of irregularly shaped lesions.  Early infection causes plant stunted and die.

Bacterial Transmission:  Typical seed-borne disease.
Bacterial Infection:  Infection occurs on leaves, stems, pods and seeds.  Seed-borne bacteria as mainly primary infection.  The secondary infection caused by rain splash. 

Reference:


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