Prunus Necrotic Ringspot Virus (PNRSV) - TAS ELISA                       Home    Products    Testing Services   Supplies

Catalog #:  V048
Source Antibody:  A rabbit polyclonal antibody for capture and a mixture of two monoclonal antibodies for detection.
Test Format:  TAS ELISA                                   

Reactivity:  This test system reacted with all PNRSV isolates tested. Reaction of the ELISA is moderately strong.  Optical Density at 405nm  is in a range of 0.600 - 2.100 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderately high.  The virus can be consistently detected in infected plant leaf tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect PNRSV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V048-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V048-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V048-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V048-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V048-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V048-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V048-C1

Coating Antibody

500

115

2 lb (1 kg)

V048-C2

Coating Antibody

1000

205

2 lb (1 kg)

V048-C3

Coating Antibody

5000

725

2 lb (1 kg)

V048-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V048-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V048-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)

V048-P1

Positive control

18

18

1 lb (0.5 kg)

V048-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase
500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase
1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase
5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instruction for TAS ELISA Reagents
To learn how to perform the Pathogen ID test: Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Prunus Necrotic Ringspot Virus
Acronym:  PNRSV
Synonyms:  Cherry (sour) necrotic ringspot virus, necrotic ringspot virus, peach ringspot virus,  prunus ringspot virus.
Group/Genus:  Ilarviruses

Vector:   
Transmission:  Mechanical, grafting, pollen.
Main host plants:  Stone fruit trees, rose.

Viral Particle:  
Virus Infection:  Prunus necrotic ringspot virus (PNRSV) infects fruit trees and causes symptoms like stunting, necrosis, chlorosis, and leaf deformities.  PNRSV is often found in rose in combination with infections of apple mosaic virus or arabis mosaic virus.  Production of virus-free stock plant is effective method to control the virus infection since the PNRSV is transmitted by grafting during plant propagation.

Reference:
1.  CMI/AAB Descriptions of Plant Viruses, No. 5.


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