Pelargonium Flower Break Virus (PFBV) - DAS ELISA                          Home    Products    Testing Services   Supplies

Catalog #:  V037
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all of the isolates of PFBV tested.  Reaction of the ELISA is relatively strong.  Optical Density at 405nm is in arrange of 0.900 - 2.200 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted at 1:810.

Specificity:  There is no obvious cross reaction found with healthy geranium tissues.  Background is relatively low on the negative control wells.

Application:  The test can be used to detect PFBV in infected geranium plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V037-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V037-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V037-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V037-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V037-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V037-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V037-C1

Coating Antibody

500

115

2 lb (1 kg)

V037-C2

Coating Antibody

1000

205

2 lb (1 kg)

V037-C3

Coating Antibody

5000

725

2 lb (1 kg)

V037-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V037-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V037-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)
V037-P1 Positive control

18

18

1 lb (0.5 kg)

V037-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELIAS
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Pelargonium Flower Break Virus
Acronym:  PFBV
Synonyms: 
Group/Genus:  Carmoviruses

Vector:  Unknown
Transmission:  Mechanical
Main host plants:  Geranium

Virus Particle:  
Virus Infection:
  Pelargonium flower break virus (PFBV) occurs widely in cultivated geraniums and are usually symptomless. Symptoms appearance in infected plants is greatly affected by various environmental factors. As the name of the virus suggests, main symptom of PFBV infection is color breaking on the flowers.  However, symptom of leaf chlorosis is also observed in some plants known to be infected with PFBV. 

Reference:
1.
CMI/AAB Descriptions of Plant Viruses, No. 130.


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