Onion Yellow Dwarf Virus (OYDV) - DAS ELISA                                  Home    Products    Testing Services   Supplies

Catalog #:  V033
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all of the isolates of OYDV tested.  Reaction of the ELISA is very strong.  Optical Density at 405nm is in a range of 2.200 - >3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted at 1:2430.

Specificity:  This test shows no cross-reaction with other potyviruses such as DsMV, LMV, LYSV, MDMV, SCMV, PepMoV, PPV, PRSV, PVV, SbMV, SYSV, TEV, TuMV, TVMV, WMV2, and ZYMV. There is no cross reaction found with healthy plant tissues.  Background is low on all of the negative control wells.

Application:  The test can be used to detect OYDV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V033-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V033-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V033-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V033-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V033-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V033-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V033-C1

Coating Antibody

500

115

2 lb (1 kg)

V033-C2

Coating Antibody

1000

205

2 lb (1 kg)

V033-C3

Coating Antibody

5000

725

2 lb (1 kg)

V033-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V033-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V033-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)

V033-P1

Positive control

18

18

1 lb (0.5 kg)

V033-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA Reagents
To learn how to perform the Pathogen ID test: Instructions for DAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Onion Yellow Dwarf Virus
Acronym:  OYDV
Synonyms:  Allium virus 1, Marmor cepae.
Group/Genus:  Potyviruses

Vector:  Aphids, including Green Peach aphid, Myzus persicae, and other aphid species.
Transmission:  Mechanical
Main host plants:  Onion, garlic leek and shallot.

Virus Particle:  Filamentous particles containing RNA genome
Virus Infection:
  Onion Yellow Dwarf Virus (OYDV) infects onion (Allium cepa), shallot (Allium cepa var. ascalonicum), garlic (Allium sativum), and leek (Allium porrum), and causes yield losses in these Allium crops.  Common symptoms of infected onion include leaf striping and plant stunting.  OYDV induces leaf mosaic in garlic and is part of the garlic mosaic virus complex.

Reference:
1. 
CMI/AAB Descriptions of Plant Viruses, No. 158.
2.  Koch, M. and Saloman, R. 1994. Serological detection of onion yellow dwarf virus in garlic. Plant Dis. 78:785-788.
3.  Lot, H., Chovelon, V., S., and Delecolle, B. 1998. Effects of onion yellow dwarf and leek yellow stripe viruses on symptomology and yield loss of three French garlic cultivars. Plant Dis. 82:1381-1385.


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