Onion Yellow Dwarf Virus (OYDV) - DAS ELISA Home Products Testing Services Supplies
Catalog #: V033
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: This test system reacted with all of the isolates of OYDV tested. Reaction of the ELISA is very strong. Optical Density at 405nm is in a range of 2.200 - >3.000 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant tissues diluted at 1:2430.
Specificity: This test shows no cross-reaction with other potyviruses such as DsMV, LMV, LYSV, MDMV, SCMV, PepMoV, PPV, PRSV, PVV, SbMV, SYSV, TEV, TuMV, TVMV, WMV2, and ZYMV. There is no cross reaction found with healthy plant tissues. Background is low on all of the negative control wells.
Application: The test can be used to detect OYDV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells | Price $US | Ship
Weight (Estimated) |
|
V033-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
210 |
2 lb (1 kg) |
|
V033-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
370 |
3 lb (1.5 kg) |
|
V033-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1450 |
12 lb (6 kg) |
| V033-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V033-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
325 |
2 lb (1 kg) |
| V033-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
505 |
3 lb (1.5 kg) |
|
V033-C1 |
Coating Antibody |
500 |
105 |
2 lb (1 kg) |
|
V033-C2 |
Coating Antibody |
1000 |
185 |
2 lb (1 kg) |
|
V033-C3 |
Coating Antibody |
5000 |
660 |
2 lb (1 kg) |
|
V033-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
130 |
2 lb (1 kg) |
|
V033-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
205 |
2 lb (1 kg) |
|
V033-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
820 |
2 lb (1 kg) |
|
V033-P1 |
Positive control |
18 |
15 |
1 lb (0.5 kg) |
|
V033-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
It is easy and convenient To Place
An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA Reagents
To learn how to perform the Pathogen ID test:
Instructions for DAS ELISA Reagents
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Onion Yellow Dwarf Virus
Acronym: OYDV
Synonyms: Allium virus 1, Marmor cepae.
Group/Genus: Potyviruses
Vector: Aphids, including Green Peach aphid, Myzus persicae,
and other aphid species.
Transmission: Mechanical
Main host plants: Onion, garlic leek and shallot.
Virus Particle: Filamentous particles containing RNA genome
Virus Infection: Onion Yellow Dwarf Virus (OYDV) infects onion (Allium
cepa), shallot (Allium cepa var. ascalonicum), garlic (Allium
sativum), and leek (Allium porrum), and causes yield losses in these
Allium crops. Common symptoms of infected onion include leaf striping and
plant stunting. OYDV induces leaf mosaic in garlic and is part of the
garlic mosaic virus complex.
Reference:
1. CMI/AAB Descriptions of Plant Viruses, No. 158.
2. Koch, M. and Saloman, R. 1994. Serological detection of
onion yellow dwarf virus in garlic. Plant Dis. 78:785-788.
3. Lot, H., Chovelon, V., S., and Delecolle, B. 1998. Effects of
onion yellow dwarf and leek yellow stripe viruses on symptomology and yield loss
of three French garlic cultivars. Plant Dis. 82:1381-1385.
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