Melon Necrotic Spot Virus (MNSV) - DAS ELISA Home Products Testing Services Supplies
Catalog #: V031
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: This test system reacted with all of the isolates or strains of MNSV tested. Reaction of the ELISA is very strong. Optical Density at 405nm is in a range of 1.200 - 2.800 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is relatively high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: This ELISA shows no cross-reaction with other carmoviruses, such as carnation mottle virus, hibiscus chlorotic ringspot virus and pelargonium flower break virus. There is no cross reaction found with healthy cucurbit plant tissues. Background is low on all of the negative control wells.
Application: The test can be used to detect MNSV in infected leaves and seeds of cucurbits.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V031-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
210 |
2 lb (1 kg) |
|
V031-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
370 |
3 lb (1.5 kg) |
|
V031-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1450 |
12 lb (6 kg) |
| V031-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V031-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
325 |
2 lb (1 kg) |
| V031-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
505 |
3 lb (1.5 kg) |
|
V031-C1 |
Coating Antibody |
500 |
105 |
2 lb (1 kg) |
|
V031-C2 |
Coating Antibody |
1000 |
185 |
2 lb (1 kg) |
|
V031-C3 |
Coating Antibody |
5000 |
660 |
2 lb (1 kg) |
|
V031-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
130 |
2 lb (1 kg) |
|
V031-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
205 |
2 lb (1 kg) |
|
V031-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
820 |
2 lb (1 kg) |
|
V031-P1 |
Positive control |
18 |
15 |
1 lb (0.5 kg) |
|
V031-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Melon Necrotic Spot Virus
Acronym: MNSV
Synonyms:
Group/Genus: Carmoviruses
Vector: Fungus
Transmission: Mechanical, seed.
Main host plants: Cucurbits
Virus Particle: Isometric particles containing RNA genome, 30 nm
in diameter.
Virus Infection: Melon necrotic spot virus (MNSV) occurs in Japan, the
Netherlands, the UK, and the USA. MNSV is transmitted by the chytrid
fungus Olpidium radicaleand in soil. This virus is also transmitted
by mechanical sap inoculation. Naturally, MNSV occurs only in greenhouse
melons and cucumbers, and causes severe yield loss. The symptoms induced
by the virus include necrotic spots or large necrotic lesions on the leaves, and
necrosis on the stems of greenhouse melons and cucumbers.
Reference:
1. CMI/AAB Descr. Pl. Viruses, No. 302, 1985.
2. Furuki, Tech. Bull. Schizuoka Agric. Exp. Stn 14:94, 1981.
3. Gonzalez-Garza, Gumpf, Kishaba and Bohn, Phytopathology 69:340,
1979.
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