Melon Necrotic Spot Virus (MNSV) - DAS ELISA                               Home    Products    Testing Services   Supplies

Catalog #:  V031
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all of the isolates or strains of MNSV tested.  Reaction of the ELISA is very strong.  Optical Density at 405_nm is in a range of 1.200 - 2.800 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted at 1:810.

Specificity:  This ELISA shows no cross-reaction with other carmoviruses, such as carnation mottle virus, hibiscus chlorotic ringspot virus and pelargonium flower break virus. There is no cross reaction found with healthy cucurbit plant tissues.  Background is low on all of the negative control wells.

Application:  The test can be used to detect MNSV in infected leaves and seeds of cucurbits.

Products: 

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA  In PDF Format  In PUB Format
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Virus

Name:  Melon Necrotic Spot Virus
Acronym: MNSV
Synonyms: 
Group/Genus:  Carmoviruses

Vector:  Fungus
Transmission:  Mechanical, seed.
Main host plants:  Cucurbits

Virus Particle:  Isometric particles containing RNA genome, 30 nm in diameter. 
Virus Infection:  Melon necrotic spot virus (MNSV) occurs in Japan, the Netherlands, the UK, and the USA.  MNSV is transmitted by the chytrid fungus Olpidium radicaleand in soil.  This virus is also transmitted by mechanical sap inoculation.  Naturally, MNSV occurs only in greenhouse melons and cucumbers, and causes severe yield loss.  The symptoms induced by the virus include necrotic spots or large necrotic lesions on the leaves, and necrosis on the stems of greenhouse melons and cucumbers.

Reference:
1.  CMI/AAB Descr. Pl. Viruses, No. 302, 1985.
2.  Furuki, Tech. Bull. Schizuoka Agric. Exp. Stn 14:94, 1981.
3.  Gonzalez-Garza, Gumpf, Kishaba and Bohn, Phytopathology 69:340, 1979.

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