Erwinia chrysanthemi (Echr) - DAS ELISA                                         Home    Products    Testing Services   Supplies

Catalog #:  B105
Source Antibody:  Rabbit polyclonal antibodies for both capture and detection.
Test Format:  DAS ELISA                                    

Reactivity:  This ELISA reacted with all Echr isolates tested.  Reaction of the ELISA is very strong.  Optical Density at 405nm  is in a range of 1.480 - 3.000 depending  on the bacterial titers in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The bacterium can be consistently detected in infected plant tissues diluted at 1:2430.

Specificity:  This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. insidiosus and C. m. subsp. nebraskensis, C. m. subsp. michiganensis, C. m. subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c. pv. diffenbachiae,  X. c. pv. pelargonii, X. c. pv. phaseoli, and X. c. pv. vesicatoria.  There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect Echr in infected host plants as well as in pure bacterial cultures.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

B105-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

B105-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

B105-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
B105-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
B105-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
B105-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

B105-C1

Coating Antibody

500

125

2 lb (1 kg)

B105-C2

Coating Antibody

1000

235

2 lb (1 kg)

B105-C3

Coating Antibody

5000

805

2 lb (1 kg)

B105-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

B105-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

B105-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

B105-P1

Positive control

18

18

1 lb (0.5 kg)

B105-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELIAS
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Bacterium

Name:  Erwinia chrysanthemi
Acronym:  Echr
Synonyms: 
Group/Genus:  Erwinia

Host plants and Diseases:  Bacterial blight of chrysanthemum, diseases in other hosts such as vegetables, cereals and woody plants.
Symptoms:  Blight, and others symptoms such as foot rot of rice, rusty canker of pear and stalk rot of corn.

Bacterial Transmission:  Infected plant residues, infested soil, agricultural equipments or activities.
Bacterial Infection:  Typical wound infection. Disease incidence increases when plants are injured by farming practices or wind.

Reference:


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