Clavibacter michiganensis pv. michiganensis (Cmm) - DAS ELISA             Home    Products    Testing Services   Supplies

Catalog #:  B103
Source Antibody:  Rabbit polyclonal antibodies for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all Cmm isolates tested.  Reaction of the ELISA is very strong.  Optical Density at 405_nm  is in a range of 1.100 - >3.000 depending  on the bacterial titers in the samples to be tested.

Sensitivity:  Sensitivity of the ELISA is high.  The bacterium can be consistently detected in infected plant leaf tissues diluted at 1:810 - 1:2430.

Specificity:  This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, E. chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c. pv. diffenbachiae,  X. c. pv. pelargonii, X. c. pv. phaseoli, and X. c. pv. vesicatoria.  This test shows cross-reaction with Clavibacter michiganensis subsp. insidiosus and C. m. subsp. nebraskensis. 
There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect Cmm in infected host plants as well as in pure bacterial cultures.

Products: 

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA  In PDF Format  In PUB Format
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Bacterium

Name:  Clavibacter michiganensis pv. michiganensis
Acronym:  Cmm
Synonyms: 
Group/Genus:  Clavibacter

Host plants and Diseases:  Bacterial canker of tomato, 
Symptoms:  Plant wilting, round spot on fruits, stems and leaves.

Bacterial Transmission:  Seeds, infected plant residues, infected stems in soil, agricultural equipments.
Bacterial Infection:  Through wounds and natural openings such as stomata and trichomes.

Reference:  

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