Clover Yellow Vein Virus - Strain Pratt (ClYVV-Pratt) - DAS ELISA            Home    Products    Testing Services   Supplies

Catalog #:  V121
Source Antibody:  Use a rabbit polyclonal antibodies made against ClYVV-strain Pratt for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system detects all known isolates of ClYVV - strain Pratt and other serological related isolates.  This test does not react with ClYVV - strain C81 and some other serological distant isolates. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in a range of 0.900 - 1.900 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues.  Background is relatively  low on the negative control wells.

Application:  The test can be used to detect ClYVV-strain Pratt and other serological related isolates in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V121-R1

ELISA Reagents, Alkaline Phosphatase

500

240

2 lb (1 kg)

V121-R2

ELISA Reagents, Alkaline Phosphatase

1000

415

3 lb (1.5 kg)

V121-R3

ELISA Reagents, Alkaline Phosphatase

5000

1660

12 lb (6 kg)
V121-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V121-K1 ELISA Kit, Alkaline Phosphatase

500

365

2 lb (1 kg)
V121-K2 ELISA Kit, Alkaline Phosphatase

1000

575

3 lb (1.5 kg)

V121-C1

Coating Antibody

500

120

2 lb (1 kg)

V121-C2

Coating Antibody

1000

215

2 lb (1 kg)

V121-C3

Coating Antibody

5000

750

2 lb (1 kg)

V121-D1

Detecting conjugate, Alkaline Phosphatase

500

155

2 lb (1 kg)

V121-D2

Detecting conjugate, Alkaline Phosphatase

1000

255

2 lb (1 kg)

V121-D3

Detecting conjugate, Alkaline Phosphatase

5000

960

2 lb (1 kg)
V121-P2 ELISA control, Positive

18

18

1 lb (0.5 kg)

V121-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Clover Yellow Vein Virus -Strain Pratt
Acronym:  ClYVV-Pratt
Synonyms:  dendrobium mosaic virus, pea mottle virus (Pratt, 1961; Johnson, 1942), pea necrosis virus (Bos et al., 1977), probably alsike clover mosaic virus, pea western ringspot virus.
Stains: There are many viral strains. CYVV-Pratt is typical isolate. Others are distantly related to the type isolates.
Group/Genus:  Potyvirus

Vector: Transmitted by a vector; an insect; Acyrthosiphon (Aulacorthum) solani, Macrosiphum euphorbiae, Myzus persicae (Singh et al., 1971); Aphididae. Transmitted in a non-persistent manner.
Transmission:
  Virus transmitted by mechanical inoculation.
Main natural host plants: Coriandrum sativum, Daucus carota (wild and domestic), Pisum sativum, Trifolium hybridum, T. incarnatum, T. pratense, T. repens, T. subterraneum, T. vesiculosum, Limonium sinuatum, Lupinus luteus, Lupinus sp., Glycine max - (Jones et al., 1977).

Virus Infection:  Symptoms include mosaics, mottles or streaks, vein yellowing or netting. Symptoms vary seasonally.
Diagnostically susceptible host species and symptoms: Chenopodium quinoa - chlorotic or necrotic local lesions; systemic mosaic and necrosis; Phaseolus vulgaris - chlorotic local lesions; systemic mosaic; Nicotiana clevelandii - chlorotic or necrotic local lesions; systemic mosaic and necrosis; N. tabacum - necrotic ringspots; not systemic; Trifolium repens - mosaic and chlorotic banding or symptomless

Geographical distribution: Probably distributed worldwide (wherever white clover occurs).

References:
1. 
Barnett, O.W., Randles, J.W. and Burrows, P.M. (1987). Phytopathology 77: 791.
2.  Hollings, M. and Stone, O.M. (1974). CMI/AAB Descr. Pl. Viruses No. 131, 4 pp.
3. 
Lawson, R.H., Brannigan, M.D. and Foster, J. (1985). Phytopathology 75: 899.


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