Clover Yellow Vein Virus - Strains C81 & Pratt (ClYVV-C81&Pratt) - DAS ELISA       Home    Products    Testing Services   Supplies

Catalog #:  V019
Source Antibody:  Use the combination of two rabbit polyclonal antibodies made against two different serotypes of ClYVV for both capture and detection in order to cover all viral isolates or strains.
Test Format:  DAS ELISA                                   

Reactivity:  This test system detects all known isolates or strains of ClYVV-strain C81 and other serological related isolates, and ClYVV-strain Pratt and other serological related isolates. Reaction of the ELISA is moderately strong.  Optical Density at 405nm  is in a range of 0.900 - 1.800 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues.  Background is relatively  low on the negative control wells.

Application:  The test can be used to detect ClYVV-strain C81 and strain Pratt and other all other isolates in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V019-R1

ELISA Reagents, Alkaline Phosphatase

500

395

2 lb (1 kg)

V019-R2

ELISA Reagents, Alkaline Phosphatase

1000

710

3 lb (1.5 kg)

V019-R3

ELISA Reagents, Alkaline Phosphatase

5000

2810

12 lb (6 kg)
V019-R4 ELISA Reagents, Alkaline Phosphatase

100

145

1 lb (0.5 kg)
V019-K1 ELISA Kit, Alkaline Phosphatase

500

565

2 lb (1 kg)
V019-K2 ELISA Kit, Alkaline Phosphatase

1000

835

3 lb (1.5 kg)

V019-C1

Coating Antibody

500

180

2 lb (1 kg)

V019-C2

Coating Antibody

1000

325

2 lb (1 kg)

V019-C3

Coating Antibody

5000

965

2 lb (1 kg)

V019-D1

Detecting conjugate, Alkaline Phosphatase

500

215

2 lb (1 kg)

V019-D2

Detecting conjugate, Alkaline Phosphatase

1000

375

2 lb (1 kg)

V019-D3

Detecting conjugate, Alkaline Phosphatase

5000

1565

2 lb (1 kg)
V019-P2 ELISA control, Positive

18

18

1 lb (0.5 kg)

V019-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Clover Yellow Vein Virus -Strains C81 and Pratt
Acronym:  ClYVV-C81&Pratt
Synonyms:  dendrobium mosaic virus, pea mottle virus (Pratt, 1961; Johnson, 1942), pea necrosis virus (Bos et al., 1977), probably alsike clover mosaic virus, pea western ringspot virus.
Stains: There are many viral strains. CYVV-Pratt is typical isolate. Others are distantly related to the type isolates.
Group/Genus:  Potyvirus

Vector: Transmitted by a vector; an insect; Acyrthosiphon (Aulacorthum) solani, Macrosiphum euphorbiae, Myzus persicae (Singh et al., 1971); Aphididae. Transmitted in a non-persistent manner.
Transmission:
  Virus transmitted by mechanical inoculation.
Main natural host plants: Coriandrum sativum, Daucus carota (wild and domestic), Pisum sativum, Trifolium hybridum, T. incarnatum, T. pratense, T. repens, T. subterraneum, T. vesiculosum, Limonium sinuatum, Lupinus luteus, Lupinus sp., Glycine max - (Jones et al., 1977).

Virus Infection:  Symptoms include mosaics, mottles or streaks, vein yellowing or netting. Symptoms vary seasonally.
Diagnostically susceptible host species and symptoms: Chenopodium quinoa - chlorotic or necrotic local lesions; systemic mosaic and necrosis; Phaseolus vulgaris - chlorotic local lesions; systemic mosaic; Nicotiana clevelandii - chlorotic or necrotic local lesions; systemic mosaic and necrosis; N. tabacum - necrotic ringspots; not systemic; Trifolium repens - mosaic and chlorotic banding or symptomless

Geographical distribution: Probably distributed worldwide (wherever white clover occurs).

References:
1. 
Barnett, O.W., Randles, J.W. and Burrows, P.M. (1987). Phytopathology 77: 791.
2.  Hollings, M. and Stone, O.M. (1974). CMI/AAB Descr. Pl. Viruses No. 131, 4 pp.
3. 
Lawson, R.H., Brannigan, M.D. and Foster, J. (1985). Phytopathology 75: 899.


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