Clover Yellow Vein Virus - Strains C81 & Pratt (ClYVV-C81&Pratt) - DAS ELISA Home Products Testing Services Supplies
Catalog #: V019
Source Antibody: Use the combination of two rabbit polyclonal
antibodies made against two different serotypes of ClYVV for both capture and detection in
order to cover all viral isolates or strains.
Test Format: DAS ELISA
Reactivity: This test system detects all known isolates or strains of ClYVV-strain C81 and other serological related isolates, and ClYVV-strain Pratt and other serological related isolates. Reaction of the ELISA is moderately strong. Optical Density at 405nm is in a range of 0.900 - 1.800 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is relatively high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues. Background is relatively low on the negative control wells.
Application: The test can be used to detect ClYVV-strain C81 and strain Pratt and other all other isolates in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V019-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
395 |
2 lb (1 kg) |
|
V019-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
710 |
3 lb (1.5 kg) |
|
V019-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
2070 |
12 lb (6 kg) |
| V019-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
135 |
1 lb (0.5 kg) |
| V019-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
495 |
2 lb (1 kg) |
| V019-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
825 |
3 lb (1.5 kg) |
|
V019-C1 |
Coating Antibody |
500 |
180 |
2 lb (1 kg) |
|
V019-C2 |
Coating Antibody |
1000 |
325 |
2 lb (1 kg) |
|
V019-C3 |
Coating Antibody |
5000 |
965 |
2 lb (1 kg) |
|
V019-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
215 |
2 lb (1 kg) |
|
V019-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
375 |
2 lb (1 kg) |
|
V019-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1105 |
2 lb (1 kg) |
| V019-P2 | ELISA control, Positive |
18 |
15 |
1 lb (0.5 kg) |
|
V019-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Clover Yellow Vein Virus -Strains C81 and Pratt
Acronym: ClYVV-C81&Pratt
Synonyms: dendrobium mosaic virus, pea mottle virus (Pratt, 1961; Johnson,
1942), pea necrosis virus (Bos et al., 1977), probably alsike clover
mosaic virus, pea western ringspot virus.
Stains: There are many viral strains. CYVV-Pratt is typical isolate.
Others are distantly related to the type isolates.
Group/Genus: Potyvirus
Vector: Transmitted by a vector; an insect; Acyrthosiphon (Aulacorthum)
solani, Macrosiphum euphorbiae, Myzus persicae (Singh et al., 1971);
Aphididae. Transmitted in a non-persistent manner.
Transmission: Virus transmitted by mechanical inoculation.
Main natural host plants: Coriandrum sativum, Daucus carota (wild
and domestic), Pisum sativum, Trifolium hybridum, T. incarnatum, T. pratense,
T. repens, T. subterraneum, T. vesiculosum, Limonium sinuatum,
Lupinus luteus, Lupinus sp., Glycine max - (Jones et al.,
1977).
Virus Infection: Symptoms include mosaics, mottles or
streaks, vein yellowing or netting. Symptoms vary seasonally.
Diagnostically susceptible host species and symptoms: Chenopodium
quinoa - chlorotic or necrotic local lesions; systemic mosaic and necrosis;
Phaseolus vulgaris - chlorotic local lesions; systemic mosaic;
Nicotiana clevelandii - chlorotic or necrotic local lesions; systemic mosaic
and necrosis; N. tabacum - necrotic ringspots; not systemic; Trifolium
repens - mosaic and chlorotic banding or symptomless
Geographical distribution: Probably distributed worldwide (wherever white clover occurs).
References:
1. Barnett, O.W., Randles, J.W. and Burrows, P.M. (1987).
Phytopathology 77: 791.
2. Hollings, M. and Stone, O.M. (1974). CMI/AAB Descr. Pl.
Viruses No. 131, 4 pp.
3. Lawson, R.H., Brannigan, M.D. and Foster, J. (1985).
Phytopathology 75: 899.