Cucumber Green Mottle Mosaic Virus (CGMMV) - DAS ELISA            Home    Products    Testing Services   Supplies

Catalog #:  V021
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all virus isolates tested.  Reaction of the ELISA is moderately strong.  Optical Density at 405nm  is in arrange of 1.000 - 1.600 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very  high.  The virus can be consistently detected in infected plant tissues diluted  at 1:2430 -  1:7290.

Specificity:  This test shows no cross-reaction with odontoglossum ringspot virus, pepper mild mottle virus and tobacco mosaic virus.  A weak cross-reaction (OD 0.200 - 0.400 at 405 nm) was found with ribgrass mosaic virus and tomato mosaic virus.  There is no cross reaction with healthy plant cucurbits  tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect CGMMV in infected cucurbit plants.

Products: 

Catalog Number

Description

Test Wells
Price $USD Ship Weight
(Estimated)

V021-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V021-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V021-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V021-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V021-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V021-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V021-C1

Coating Antibody

500

115

2 lb (1 kg)

V021-C2

Coating Antibody

1000

205

2 lb (1 kg)

V021-C3

Coating Antibody

5000

725

2 lb (1 kg)

V021-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V021-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V021-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)
VS021-LF1 ASPOT√CHECK LF™, lateral flow device, complete kit for real-time quick assay 10 105 2 lb (1 kg)
VS021-LF2 ASPOT√CHECK LF™, lateral flow device, complete kit for real-time quick assay 100 850 5 lb (2 kg)
V021-P1 Positive control

18

20

1 lb (0.5 kg)

V021-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000  130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000  400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Cucumber Green Mottle Mosaic Virus
Acronym:  CGMMV
Synonyms:  Bottlegourd indian mosaic virus, cucumber virus 2, 3 and 4, .
Group/Genus:  Tobamoviruses

Vector:  Unknown
Transmission:  Seed, mechanical.
Main host plants:  Cucurbits.

Viral Particle:  Rod-shaped, 300 x 18 nm, .containing RNA genome.
Virus Infection:   Cucumber green mottle mosaic virus (CGMMV) causes leaf mottling, blistering and distortion with stunted growth in cucumber. Cucumber fruits are usually symptomless.  In watermelon, CGMMV induces slight leaf mottling and dwarfing, but can cause serious internal discoloration and decomposition of the fruit. The virus is readily transmitted by mechanical methods such as foliage contact, handling of the plants during cultivation, soil contamination.  It is also transmitted through infected seeds.  CGMMV is found in Europe, Asia and other areas in the world.

References:
1. 
CMI/AAB Descriptions of Plant Viruses, No. 154.
2.  Ainsworth, Ann. appl. Biol. 22:55, 1935. Hollings, Komuro and Tochihara, CMI/AAB Descr. Pl. Viruses, No. 154, 1975. Rao and Varma, Phytopath. Z. 109:325, 1984.


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