Broad Bean Wilt Virus - 2 (BBWV-2) - DAS ELISA          Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V212
Source Antibody:  Use a rabbit polyclonal antibodies made against BBWV-2 for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system detects all known isolates of BBWV-2 and other serological related isolates.  This test does not react with BBWV-1 and some other serological distant isolates. Reaction of the ELISA is strong.  Optical Density at 405_nm  is in a range of 0.900 - 3.120 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 2430.

Specificity:  There is no cross reaction with healthy plant tissues. Background is moderately low on the negative control wells.

Application:  The test can be used to detect viral isolates of BBWV-2 in infected plants.

Products: 

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To know more about the products:  Product Items
To learn how to perform the test: Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Broad Bean Wilt Virus -2
Acronym:  BBWV-2
Synonyms:  catalpa chlorotic leaf spot virus (Schmelzer, 1970), tropaeolum ringspot virus (Cook and Gibbs, 1971), nasturtium ringspot virus, Ringmosaikvirus der Kapuzinerkresse (Frowd and Tomlinson, 1972), petunia ringspot virus (Hull and Plaskitt, 1973; 1974), P.O. pea streak virus, parsley virus 3.
Stains: BBWV-1, BBWV-2.
Group/Genus:  Fabavirus

Vector: Transmitted by a vector; an insect; Acyrthosiphon pisum, Aphis craccivora, A. faba, A. nasturtii, Macrosiphum euphorbiae, M. solanifolii, M. persicae; Aphididae. Transmitted in a non-persistent manner.
Transmission:  Virus transmitted by mechanical inoculation; not transmitted by seed.
Main natural host plants: Vicia faba, Pisum sativum, Spinacia oleracea, Petroselinum crispum, Tropaeolum majus, Petunia × hybrida, Plantago lanceolata.

Virus Infection:  Symptoms include vein clearing, mottling and necrosis of shoot apex, plant wilts, wilting, mottled, mosaic, ringspots, malformed and stunted. Symptoms vary seasonally and vary in different host plants.
Diagnostically susceptible host species and symptoms: Chenopodium amaranticolor, C. quinoa - chlorotic local lesions; tip epinasty, leaves malformed and mottled; Spinacia oleracea - systemic wilt, necrosis and die-back; Vicia faba - apical necrosis, wilting and mosaic; Vigna unguiculata cv. Blackeye - red or chlorotic local lesions; systemic mosaic and malformation.

Geographical distribution: Spreads in the African region, the Eurasian region, the Middle East, the North American region, and the Pacific region; Australia and China. Found, but with no evidence of spread, in Argentina.

References:
1.  Govier, D.A. (1988). Ann. appl. Biol. 113: 287.
2.  Taylor, R.H. and Stubbs, L.L. (1972). CMI/AAB Descr. Pl. Viruses No. 81, 4 pp.
3.  Xu, Z.G., Cockbain, A.J., Woods, R.D. and Govier, D.A. (1988). Ann. appl. Biol. 113: 287.

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