Zucchini Green Mottle Mosaic Virus (ZGMMV) - DAS ELISA        Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V159
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all ZGMMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.750 - >2.700 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  This test system reacts negatively with tobamoviruses such as CGMMV, KGMMV, ORSV, RMV, SHMV and TMV. This ELISA shows some cross-reactivity with PMMoV and ToMV at low levels (0.300 - 0.400 of O.D.405nm ). The test does not cross-react with other tobamoviruses such as CGMMV, ORSV, RMV and TMV.   There is no cross reaction with healthy plant  tissues such as zucchini or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect ZGMMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V159-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V159-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V159-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V159-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V159-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V159-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V159-C1

Coating Antibody

500

125

2 lb (1 kg)

V159-C2

Coating Antibody

1000

235

2 lb (1 kg)

V159-C3

Coating Antibody

5000

805

2 lb (1 kg)

V159-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V159-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V159-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V159-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V159-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Zucchini Green Mottle Mosaic Virus
Acronym:  ZGMMV
Synonyms:  
Group/Genus:  Tobamovirus

Vector:  Non-Vector Transmission
Transmission: Virus is transmitted by seeds.
Main natural host plants: Zucchini.

Virus Infection:  Symptoms are expressed in the leaf and infructescence; include stunting, deformation, and reduced vigor; include stunting of all plant parts; include deformation of leaves and fruits. Symptoms in leaves include size reduction and development of patterns or markings. Leaf margin can be chlorotic and changed in color. Leaves with mosaic and mottle have green mosaic and green mottle. Fruit symptoms include malformation, size reduction, and change in texture.
Diagnostically susceptible host species and symptoms: Datura stramonium necrotic local lesion. Infection is local. Symptoms visible; after 3 days. Local symptoms include necrotic spots. Local lesions found on leaf. Lesions are necrotic. Diagnostically insusceptible host species: Nicotiana tabacum.

Geographical distribution: Geographical distribution of the virus is probably restricted. The virus occurs in South Korea.

References:
1. 
Ryu,K.H., Min,B.E., Choi,G.S., Choi,S.H., Kwon,S.B., Noh,G.M., Yoon,J.Y., Choi,Y.M., Jang,S.H., Lee,G.P., Cho,K.H. and Park,W.M. (2000) Zucchini green mottle mosaic virus is a new tobamovirus; comparison of its coat protein gene with that of kyuri green mottle mosaic virus. Arch. Virol. 145, 2325-2333.


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