Zucchini Green Mottle Mosaic Virus (ZGMMV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V159
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all ZGMMV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.750 - >2.700 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: This test system reacts negatively with tobamoviruses such as CGMMV, KGMMV, ORSV, RMV, SHMV and TMV. This ELISA shows some cross-reactivity with PMMoV and ToMV at low levels (0.300 - 0.400 of O.D.405nm ). The test does not cross-react with other tobamoviruses such as CGMMV, ORSV, RMV and TMV. There is no cross reaction with healthy plant tissues such as zucchini or other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect ZGMMV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells | Price $US |
Ship
Weight (Estimated) |
|
V159-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
250 |
2 lb (1 kg) |
|
V159-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
425 |
3 lb (1.5 kg) |
|
V159-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1690 |
12 lb (6 kg) |
| V159-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V159-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
385 |
2 lb (1 kg) |
| V159-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
610 |
3 lb (1.5 kg) |
|
V159-C1 |
Coating Antibody |
500 |
125 |
2 lb (1 kg) |
|
V159-C2 |
Coating Antibody |
1000 |
235 |
2 lb (1 kg) |
|
V159-C3 |
Coating Antibody |
5000 |
805 |
2 lb (1 kg) |
|
V159-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
170 |
2 lb (1 kg) |
|
V159-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
285 |
2 lb (1 kg) |
|
V159-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
980 |
2 lb (1 kg) |
|
V159-P2 |
ELISA control, positive |
18 |
15 |
1 lb (0.5 kg) |
|
V159-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 |
2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
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Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Zucchini Green Mottle Mosaic Virus
Acronym: ZGMMV
Synonyms:
Group/Genus: Tobamovirus
Vector: Non-Vector Transmission
Transmission: Virus is transmitted by seeds.
Main natural host plants: Zucchini.
Virus Infection: Symptoms are expressed in the leaf and
infructescence; include stunting, deformation, and reduced vigor; include
stunting of all plant parts; include deformation of leaves and fruits. Symptoms
in leaves include size reduction and development of patterns or markings. Leaf
margin can be chlorotic and changed in color. Leaves with mosaic and mottle have
green mosaic and green mottle. Fruit symptoms include malformation, size
reduction, and change in texture.
Diagnostically susceptible host species and symptoms: Datura
stramonium — necrotic local lesion. Infection is local. Symptoms visible;
after 3 days. Local symptoms include necrotic spots. Local lesions found on
leaf. Lesions are necrotic. Diagnostically insusceptible host species:
Nicotiana tabacum.
Geographical distribution: Geographical distribution of the virus is probably restricted. The virus occurs in South Korea.
References:
1. Ryu,K.H., Min,B.E., Choi,G.S., Choi,S.H., Kwon,S.B., Noh,G.M.,
Yoon,J.Y., Choi,Y.M., Jang,S.H., Lee,G.P., Cho,K.H. and Park,W.M. (2000)
Zucchini green mottle mosaic virus is a new tobamovirus; comparison of its coat
protein gene with that of kyuri green mottle mosaic virus. Arch. Virol.
145, 2325-2333.