Yam Mosaic Virus (YMV) - DAS ELISA                                              Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V183
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all YMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.750 - 2.850 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as rice and other host plants. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect YMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V183-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V183-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V183-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V183-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V183-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V183-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V183-C1

Coating Antibody

500

125

2 lb (1 kg)

V183-C2

Coating Antibody

1000

235

2 lb (1 kg)

V183-C3

Coating Antibody

5000

805

2 lb (1 kg)

V183-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V183-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V183-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V183-P2

ELISA Control, Positive

18

18

1 lb (0.5 kg)

V183-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Yam Mosaic Virus
Acronym:  YMV
Synonyms: clover mosaic virus, pea wilt virus (Bos et al., 1959; Johnson, 1942)
Group/Genus: Potyvirus

Vector:  Transmitted by a vector; an insect; Aphis craccivora, A. gossypii, Rhopalosiphum maidis, Toxoptera citricidus; Aphididae. Transmitted in a non-persistent manner.
Transmission: Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed (is by vegetative propagation).
Main host plants: Dioscorea alata, D. cayenensis, D. dumetorum, D. praehensilis, D. preussii, D. rotundata.

Virus Infection:  Cause symptoms of mosaic, vein banding. Symptoms disappear soon after infection.
Diagnostically susceptible host species and symptoms:
Nicotiana benthamiana - faint systemic chlorosis and mosaic; Dioscorea cayenensis, D. praehensilis - systemic mosaic and vein banding.

Geographical distribution: Spreads in the African region (in the west); Guadeloupe and Guyana.

 References:
1. 
Thouvenel, J.C. and Fauquet, C. (1979). Ann. appl. Biol. 93: 279.
2.  Thouvenel, J.-C. and Fauquet, C. (1986). AAB Descr. Pl. Viruses No. 314, 4 pp.


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