Catalog #: B149
Reactivity: This ELISA reacted with all Xab isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in a range of 1.000 - 2.700 depending on the bacterial titers in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The bacterium can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.
Specificity: This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis, C. m. subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, Erwinia chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c. pv. dieffenbachiae, X. c. pv. pelargonii, X. c. pv. phaseoli, and X. c. pv. vesicatoria. There is no cross reaction with healthy plant tissues. Background is very low on all of the negative control wells.
Application: The test can be used to detect Xab in infected host plants as well as in pure bacterial cultures.
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Information About the Bacterium
Name: Xanthomonas axonopodis pv.
Host plants and Diseases:
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