Clover Wound Tumor Virus (WTV) - DAS ELISA                      Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V225
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all WTV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.830 - 2.760 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as clover and other host plants. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect WTV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)
V225-R1 ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)
V225-R2 ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)
V225-R3 ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V225-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V225-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V225-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)
V225-C1 Coating Antibody

500

125

2 lb (1 kg)
V225-C2 Coating Antibody

1000

235

2 lb (1 kg)
V225-C3 Coating Antibody

5000

805

2 lb (1 kg)
V225-D1 Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)
V225-D2 Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)
V225-D3 Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V225-P2 ELISA Control, Positive

18

18

1 lb (0.5 kg)
V225-N1 Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Clover Wound Tumor Virus
Acronym:  WTV
Synonyms: wound tumor virus, clover big vein virus, Aureogenus magnivena virus, Trifoliumvirus nervicrassans virus
Group/Genus:  Phytoreovirus

Vector:  Transmitted by a vector; an insect; Agalliopsis novella, Agallia constricta, A. quadripunctata; Cicadellidae. Not transmitted by Aceratagallia sanguinolenta. Transmitted in a persistent manner. Virus retained when the vector moults; multiplies in the vector; transmitted congenitally to the progeny of the vector (2% of vector eggs). not transmitted by mechanical inoculation; not transmitted by seed.
Transmission: Not transmitted by mechanical inoculation; not transmitted by seed.
Main natural host plants: Trifolium incarnatum, Melilotus officinalis.

Virus Infection: Causes irregular vein enlargement on the abaxial surface of leaves, depression of veins on the adaxial surface, root tumours at bases of lateral roots. Symptoms persist.
Diagnostically susceptible host species and symptoms:
Trifolium incarnatum - systemic infection, and quantitative assays using vector cell monolayers.

Geographical distribution: Spreads in the North American region.

References:
1. 
Black, L.M. (1970). CMI/AAB Descr. Pl. Viruses No. 34, 4 pp.
2.  Hillman, B.I., Anzola, J.V., Halpern, B.T., Cavileer, T.D. and Nuss, D.L. (1991). Virology 185: 896.


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