Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Clover Wound Tumor Virus (WTV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V225
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all WTV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.830 - 2.760 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as clover and other host plants. Background is relative low on all of the negative control wells.
Application: The test can be used to detect WTV in infected host plants.
Products:
|
Catalog Number |
Description | Test Wells | Price $US |
Ship
Weight (Estimated) |
| V225-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
240 |
2 lb (1 kg) |
| V225-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
415 |
3 lb (1.5 kg) |
| V225-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1660 |
12 lb (6 kg) |
| V225-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V225-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
355 |
2 lb (1 kg) |
| V225-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
565 |
3 lb (1.5 kg) |
| V225-C1 | Coating Antibody |
500 |
120 |
2 lb (1 kg) |
| V225-C2 | Coating Antibody |
1000 |
215 |
2 lb (1 kg) |
| V225-C3 | Coating Antibody |
5000 |
750 |
2 lb (1 kg) |
| V225-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
155 |
2 lb (1 kg) |
| V225-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
255 |
2 lb (1 kg) |
| V225-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
960 |
2 lb (1 kg) |
| V225-P2 | ELISA Control, Positive |
18 |
15 |
1 lb (0.5 kg) |
| V225-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Clover Wound Tumor Virus
Acronym: WTV
Synonyms: wound tumor virus, clover big vein virus, Aureogenus
magnivena virus, Trifoliumvirus nervicrassans virus
Group/Genus: Phytoreovirus
Vector: Transmitted by a vector; an insect; Agalliopsis novella,
Agallia constricta, A. quadripunctata; Cicadellidae. Not transmitted by
Aceratagallia sanguinolenta. Transmitted in a persistent manner. Virus
retained when the vector moults; multiplies in the vector; transmitted
congenitally to the progeny of the vector (2% of vector eggs). not transmitted
by mechanical inoculation; not transmitted by seed.
Transmission: Not transmitted by mechanical inoculation; not transmitted
by seed.
Main natural host plants: Trifolium incarnatum, Melilotus officinalis.
Virus Infection: Causes irregular vein enlargement on the abaxial
surface of leaves, depression of veins on the adaxial surface, root tumours at
bases of lateral roots. Symptoms
persist.
Diagnostically susceptible host species and symptoms: Trifolium
incarnatum - systemic infection, and quantitative assays using vector cell
monolayers.
References:
1. Black, L.M. (1970). CMI/AAB Descr. Pl. Viruses No. 34, 4 pp.
2. Hillman, B.I., Anzola, J.V., Halpern, B.T., Cavileer, T.D. and Nuss,
D.L. (1991). Virology 185: 896.