Watermelon Silver Mottle Virus (WSMoV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V172
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with the WSMoV isolates from India or Far East which do not react with other specific antibodies against TSWV and INSV. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.900 - >2.500 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as watermelon or other cucurbit host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect WSMoV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V172-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
210 |
2 lb (1 kg) |
|
V172-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
370 |
3 lb (1.5 kg) |
|
V172-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1450 |
12 lb (6 kg) |
| V172-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V172-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
325 |
2 lb (1 kg) |
| V172-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
505 |
3 lb (1.5 kg) |
|
V172-C1 |
Coating Antibody |
500 |
105 |
2 lb (1 kg) |
|
V172-C2 |
Coating Antibody |
1000 |
185 |
2 lb (1 kg) |
|
V172-C3 |
Coating Antibody |
5000 |
660 |
2 lb (1 kg) |
|
V172-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
130 |
2 lb (1 kg) |
|
V172-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
205 |
2 lb (1 kg) |
|
V172-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
820 |
2 lb (1 kg) |
|
V172-P2 |
ELISA control, positive |
18 |
15 |
1 lb (0.5 kg) |
|
V172-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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Items
To learn how to perform the test: Instructions for DAS
ELIAS
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Watermelon Silver Mottle Virus
Acronym: WSMoV
Synonyms: watermelon spotted
wilt virus
Group/Genus: Tospovirus
Vector: Transmitted by a vector; an insect; Thrips tabaci, T.
setosus, T. parmi, Frankliniella schultzei, F. occidentalis, F. fusca and
Scirtothrips dorsalis; Thysanoptera. Transmitted in a persistent manner.
Virus retained when the vector moults; multiplies in the vector (probably); not
transmitted congenitally to the progeny of the vector.
Transmission: transmitted by mechanical inoculation; transmitted by
grafting; not transmitted by contact between plants; not transmitted by seed;
not transmitted by pollen.
Main natural host plants:
Virus Infection:
Susceptible hosts and symptoms:
Geographical distribution:
References:
1. Yeh S.D., Chang T.F. (1995). Nucleotide sequence of the N gene of
watermelon silver mottle virus, a proposed new member of the genus Tospovirus.
Phytopathology 85:58-64(1995).