Wheat Dwarf monogeminivirus (WDV) - DAS ELISA Home Products Testing Services Supplies
Catalog #: V227
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: This test system reacted with all
WDV isolates tested. Reaction of the ELISA is relative strong. Optical Density at 405nm
is in a range of 0.850 - 2.260 depending on the virus titer in the
samples tested.
Sensitivity: Sensitivity of the ELISA is relative high. The virus can be consistently detected in infected plant leaf tissues diluted at 1:270 - 810.
Specificity: There is no cross reaction with healthy plant tissues. Background is low on the negative control wells.
Application: The test can be used to detect WDV in infected host plans.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V227-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
250 |
2 lb (1 kg) |
|
V227-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
425 |
3 lb (1.5 kg) |
|
V227-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1690 |
12 lb (6 kg) |
| V227-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V227-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
385 |
2 lb (1 kg) |
| V227-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
610 |
3 lb (1.5 kg) |
|
V227-C1 |
Coating Antibody |
500 |
125 |
2 lb (1 kg) |
|
V227-C2 |
Coating Antibody |
1000 |
235 |
2 lb (1 kg) |
|
V227-C3 |
Coating Antibody |
5000 |
805 |
2 lb (1 kg) |
|
V227-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
170 |
2 lb (1 kg) |
|
V227-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
285 |
2 lb (1 kg) |
|
V227-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
980 |
2 lb (1 kg) |
|
V227-P1 |
Positive control |
18 |
15 |
1 lb (0.5 kg) |
|
V227-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
It is easy and convenient To Place
An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions for DAS
ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Wheat Dwarf Monogeminivirus
Acronym: WDV
Synonyms:
Viral Family: Geminiviridae
Vector: Transmitted by a vector; an insect; Psammotettix alienus;
Cicadellidae. Not transmitted by Javesella pellucida, Laodelphax striatellus,
Macrosteles laevis. Transmitted in a persistent manner. Virus retained when
the vector moults; does not multiply in the vector; not transmitted congenitally
to the progeny of the vector.
Transmission: Virus not transmitted by mechanical inoculation; not
transmitted by contact between plants; not transmitted by seed; not transmitted
by pollen.
Main natural host plants and symptoms: Virus infect barley and
wheat. Symptoms include yellowing and dwarfing. Symptoms persist. .
Diagnostically susceptible host species and symptoms: Avena sativa - yellowing and dwarfing; Bromus secalinus - yellowing, plants die prematurely; Hordeum vulgare - dwarfing, poor heads and yellowing.
Geographical distribution: Spreads in Bulgaria, the former Czechoslovakia, France, Hungary, and the former USSR. Found, but with no evidence of spread, in Sweden.
References:
1. Lindsten, K. (1991). In: Proc. 6th Conf. on Virus Diseases of
Gramineae in Europe, Torino, June 1991.
2. Matzeit, V., Laufs, J., Kammann, M., Schaefer, S., Schalk,
H.-J., Schmidt, B., Wirtz, U., Baker, B. and Gronenborn, B. (1990). Monograph
Series, Plant Biology 11: 71.