Wheat Dwarf monogeminivirus (WDV) - DAS ELISA                                                Home    Products    Testing Services   Supplies

Catalog #:  V227
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all WDV isolates tested. Reaction of the ELISA is relative strong.  Optical Density at 405nm  is in a range of 0.850 - 2.260 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relative high.  The virus can be consistently detected in infected plant leaf tissues diluted at 1:270 - 810.

Specificity:  There is no cross reaction with healthy plant tissues.  Background is low on the negative control wells.

Application:  The test can be used to detect WDV in infected host plans.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V227-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V227-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V227-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V227-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V227-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V227-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V227-C1

Coating Antibody

500

125

2 lb (1 kg)

V227-C2

Coating Antibody

1000

235

2 lb (1 kg)

V227-C3

Coating Antibody

5000

805

2 lb (1 kg)

V227-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V227-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V227-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V227-P1

Positive control

18

18

1 lb (0.5 kg)

V227-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
Instructions for DAS ELISA 
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Wheat Dwarf Monogeminivirus
Acronym:  WDV
Synonyms:  
Viral Family: Geminiviridae

Vector:  Transmitted by a vector; an insect; Psammotettix alienus; Cicadellidae. Not transmitted by Javesella pellucida, Laodelphax striatellus, Macrosteles laevis. Transmitted in a persistent manner. Virus retained when the vector moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector.
Transmission: Virus not transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main natural host plants and symptoms: Virus infect barley and wheat. Symptoms include yellowing and dwarfing. Symptoms persist. .

Diagnostically susceptible host species and symptoms: Avena sativa - yellowing and dwarfing; Bromus secalinus - yellowing, plants die prematurely; Hordeum vulgare - dwarfing, poor heads and yellowing.

Geographical distribution: Spreads in Bulgaria, the former Czechoslovakia, France, Hungary, and the former USSR. Found, but with no evidence of spread, in Sweden.

References:
1. 
Lindsten, K. (1991). In: Proc. 6th Conf. on Virus Diseases of Gramineae in Europe, Torino, June 1991.
2.  Matzeit, V., Laufs, J., Kammann, M., Schaefer, S., Schalk, H.-J., Schmidt, B., Wirtz, U., Baker, B. and Gronenborn, B. (1990). Monograph Series, Plant Biology 11: 71.


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