Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Velvet Tobacco Mottle Virus (VTMoV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V224
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all VTMoV isolates tested. Reaction of the ELISA is strong. Optical Density at 405nm is in arrange of 0.960 - >3.000 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as velvet tobacco and other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect VTMoV in infected host plants.
Products:
|
Catalog Number |
Description | Test Wells | Price $US |
Ship
Weight (Estimated) |
| V224-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
240 |
2 lb (1 kg) |
| V224-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
415 |
3 lb (1.5 kg) |
| V224-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1660 |
12 lb (6 kg) |
| V224-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V224-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
355 |
2 lb (1 kg) |
| V224-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
565 |
3 lb (1.5 kg) |
| V224-C1 | Coating Antibody |
500 |
120 |
2 lb (1 kg) |
| V224-C2 | Coating Antibody |
1000 |
215 |
2 lb (1 kg) |
| V224-C3 | Coating Antibody |
5000 |
750 |
2 lb (1 kg) |
| V224-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
155 |
2 lb (1 kg) |
| V224-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
255 |
2 lb (1 kg) |
| V224-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
960 |
2 lb (1 kg) |
| V224-P2 | ELISA Control, Positive |
18 |
15 |
1 lb (0.5 kg) |
| V224-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Velvet Tobacco Mottle Virus
Acronym: VTMoV
Synonyms:
Group/Genus: Sobemovirus
Vector: Transmitted by a vector; an insect; Cyrtopeltis nicotianae:
Miridae. Transmitted in a semi-persistent manner (atypically). Virus does not
multiply in the vector; does not require a helper virus for vector transmission
(Gibb and Randles, 1988).
Transmission: Transmitted by mechanical inoculation; not transmitted by
seed.
Main natural host plants: Nicotiana velutina
Virus Infection: Causes systemic mosaic and leaf malformation. Symptoms
persist.
Diagnostically susceptible host species and symptoms: Nicotiana
clevelandii - chlorotic to necrotic local lesions, then systemic vein
yellowing and mosaic; Nicotiana glutinosa, N. × edwardsonii - mild
systemic mosaic.
References:
1. Gibb, K.S. and Randles, J.W. (1990). Ann. appl. Biol. 116:
513.
2. Randles, J.W. and Francki, R.I.B. (1986). CMI/AAB Descr. Pl.
Viruses No. 317, 5 pp.