Velvet Tobacco Mottle Virus (VTMoV) - DAS ELISA                      Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V224
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all VTMoV isolates tested. Reaction of the ELISA is strong.  Optical Density at 405nm  is in arrange of 0.960 - >3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as velvet tobacco and other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect VTMoV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)
V224-R1 ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)
V224-R2 ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)
V224-R3 ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V224-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V224-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V224-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)
V224-C1 Coating Antibody

500

125

2 lb (1 kg)
V224-C2 Coating Antibody

1000

235

2 lb (1 kg)
V224-C3 Coating Antibody

5000

805

2 lb (1 kg)
V224-D1 Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)
V224-D2 Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)
V224-D3 Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V224-P2 ELISA Control, Positive

18

18

1 lb (0.5 kg)
V224-N1 Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Velvet Tobacco Mottle Virus
Acronym:  VTMoV
Synonyms: 
Group/Genus:  Sobemovirus

Vector:  Transmitted by a vector; an insect; Cyrtopeltis nicotianae: Miridae. Transmitted in a semi-persistent manner (atypically). Virus does not multiply in the vector; does not require a helper virus for vector transmission (Gibb and Randles, 1988).
Transmission: Transmitted by mechanical inoculation; not transmitted by seed.
Main natural host plants: Nicotiana velutina

Virus Infection: Causes systemic mosaic and leaf malformation. Symptoms persist.
Diagnostically susceptible host species and symptoms:
Nicotiana clevelandii - chlorotic to necrotic local lesions, then systemic vein yellowing and mosaic;  Nicotiana glutinosa, N. edwardsonii - mild systemic mosaic.

Geographical distribution:

References:
1. 
Gibb, K.S. and Randles, J.W. (1990). Ann. appl. Biol. 116: 513.
2.  Randles, J.W. and Francki, R.I.B. (1986). CMI/AAB Descr. Pl. Viruses No. 317, 5 pp.


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