Tobacco Etch Virus (TEV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V140
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all TEV isolates tested. Reaction of the ELISA is strong. Optical Density at 405nm is in arrange of 1.200 - >2.900 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as tobacco, tomato or other host plants. Background is very low on all of the negative control wells.
Application: The test can be used to detect TEV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V140-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
210 |
2 lb (1 kg) |
|
V140-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
370 |
3 lb (1.5 kg) |
|
V140-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1450 |
12 lb (6 kg) |
| V140-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V140-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
325 |
2 lb (1 kg) |
| V140-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
505 |
3 lb (1.5 kg) |
|
V140-C1 |
Coating Antibody |
500 |
105 |
2 lb (1 kg) |
|
V140-C2 |
Coating Antibody |
1000 |
185 |
2 lb (1 kg) |
|
V140-C3 |
Coating Antibody |
5000 |
660 |
2 lb (1 kg) |
|
V140-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
130 |
2 lb (1 kg) |
|
V140-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
205 |
2 lb (1 kg) |
|
V140-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
820 |
2 lb (1 kg) |
|
V140-P1 |
Positive control |
18 |
15 |
1 lb (0.5 kg) |
|
V140-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Tobacco Etch Virus
Acronym: TEV
Synonyms: datura Z virus, tobacco severe etch virus, tomato etch
virus
Group/Genus: Potyvirus
Vector: Transmitted by a vector; an insect; more than 10 spp.,
especially Myzus persicae; Aphididae. Transmitted in a non-persistent
manner. Virus does not require a helper virus for vector transmission.
Transmission: Virus transmitted by mechanical inoculation; not
transmitted by seed.
Main host plants: Many natural host plants including Cassia
obtusifolia, Capsicum annuum, Datura stramonium, Lycopersicon esculentum,
Nicotiana tabacum, and Physalis spp.
Virus Infection: Symptoms persist and may be
strain-dependant. Symptoms varies, including necrotic leaf etching, leaf
mottling and distortion, mosaic, vein banding, malformation and necrosis,
stunting and reduced seed yield.
Diagnostically susceptible host species and symptoms: Chenopodium
album, C. amaranticolor and C. quinoa - local chlorotic or necrotic
lesions, not systemic; Datura stramonium - systemic mosaic, ringspots,
rugosity and leaf malformation
Geographical distribution: Spreads in the Eastern Asian region and the South and Central American region; Canada, China, Mexico, Puerto Rico, Venezuela, and the USA.
References:
1. Dougherty, W.G. and Hiebert, E. (1980). Virology 101:
466.
2. Purcifull, D.E. and Hiebert, E. (1982). CMI/AAB Descr. Pl.
Viruses No. 258, 7 pp.