Tobacco Etch Virus (TEV) - DAS ELISA                              Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V140
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all TEV isolates tested. Reaction of the ELISA is strong.  Optical Density at 405nm  is in arrange of 1.200 - >2.900 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as tobacco, tomato or other host plants. Background is very low on all of  the negative control wells.

Application:  The test can be used to detect TEV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $US Ship Weight
(Estimated)

V140-R1

ELISA Reagents, Alkaline Phosphatase

500

220

2 lb (1 kg)

V140-R2

ELISA Reagents, Alkaline Phosphatase

1000

385

3 lb (1.5 kg)

V140-R3

ELISA Reagents, Alkaline Phosphatase

5000

1515

12 lb (6 kg)
V140-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V140-K1 ELISA Kit, Alkaline Phosphatase

500

330

2 lb (1 kg)
V140-K2 ELISA Kit, Alkaline Phosphatase

1000

520

3 lb (1.5 kg)

V140-C1

Coating Antibody

500

110

2 lb (1 kg)

V140-C2

Coating Antibody

1000

195

2 lb (1 kg)

V140-C3

Coating Antibody

5000

690

2 lb (1 kg)

V140-D1

Detecting conjugate, Alkaline Phosphatase

500

135

2 lb (1 kg)

V140-D2

Detecting conjugate, Alkaline Phosphatase

1000

215

2 lb (1 kg)

V140-D3

Detecting conjugate, Alkaline Phosphatase

5000

860

2 lb (1 kg)

V140-P1

Positive control

18

20

1 lb (0.5 kg)

V140-N1

Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
90
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
120
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
350
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Tobacco Etch Virus
Acronym:  TEV
Synonyms:  datura Z virus, tobacco severe etch virus, tomato etch virus
Group/Genus:  Potyvirus

Vector:  Transmitted by a vector; an insect; more than 10 spp., especially Myzus persicae; Aphididae. Transmitted in a non-persistent manner. Virus does not require a helper virus for vector transmission.
Transmission: Virus transmitted by mechanical inoculation; not transmitted by seed.
Main host plants: Many natural host plants including Cassia obtusifolia, Capsicum annuum, Datura stramonium, Lycopersicon esculentum, Nicotiana tabacum,  and Physalis spp.

Virus Infection:  Symptoms persist and may be strain-dependant. Symptoms varies, including necrotic leaf etching, leaf mottling and distortion, mosaic, vein banding, malformation and necrosis, stunting and reduced seed yield.
Diagnostically susceptible host species and symptoms: Chenopodium album, C. amaranticolor and C. quinoa - local chlorotic or necrotic lesions, not systemic; Datura stramonium - systemic mosaic, ringspots, rugosity and leaf malformation

Geographical distribution: Spreads in the Eastern Asian region and the South and Central American region; Canada, China, Mexico, Puerto Rico, Venezuela, and the USA.

References:
1. 
Dougherty, W.G. and Hiebert, E. (1980). Virology 101: 466.
2.  Purcifull, D.E. and Hiebert, E. (1982). CMI/AAB Descr. Pl. Viruses No. 258, 7 pp.


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