Sowbane Mosaic Virus (SoMV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V157
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all SoMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405_nm  is in arrange of 0.850 - >2.900 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as sowbane or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect SoMV in infected host plants.

Products: 

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To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Sowbane Mosaic Virus
Acronym:  SoMV
Synonyms:  apple latent virus 2 (Bancroft and Tolin, 1967; Kirkpatrick et al., 1965), chenopodium mosaic virus (Bennett and Costa, 1961; Dias and Waterworth, 1967; Silva et al., 1958; Vuittenez, 1962), chenopodium seed-borne mosaic virus (Dias and Waterworth, 1967), chenopodium star mottle virus (Bancroft and Tolin, 1967)
Group/Genus:  Sobemovirus

Vector:  Transmitted by probably a vector; an insect; Myzus persicae, Circulifer tenellus, Liriomyza langei, Halticus citri (Bennett and Costa, 1961). Probably none of these transmit frequently or specifically; Aphididae, or Cicadellidae, or Coleoptera (and Diptera). Not transmitted by Myzus persicae; Dias and Waterworth (1967). Transmitted in a non-persistent manner (Bennett and Costa, 1961). .
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; transmitted by seed (up to 60% in Chenopodium quinoa; Dias and Waterworth, 1967, and 20-70% in Chenopodium murale; Bennett and Costa, 1961); transmitted by pollen to the seed and transmitted by pollen to the pollinated plant (pollen contains much virus, Francki and Miles, 1985).
Main natural host plants: Chenopodium spp.; Vitis sp., Prunus domestica; Atriplex suberecta.

Virus Infection:  causes fsystemic chlorotic mottling (Chenopodium spp.). latent infection, but rarely infected (Vitis sp., Prunus domestica). stunting and leaf deformation (Atriplex suberecta). Symptoms persist.
Diagnostically susceptible host species and symptoms: Chenopodium amaranticolor, C. quinoa, C. murale - chlorotic local lesions, systemic yellow flecking, star shaped patterns, leaf deformation. Beta vulgaris - mild systemic mosaic.

Geographical distribution: Spreads in the South and Central American region; Australia, Bulgaria, Canada, the former Czechoslovakia, Italy, Japan, the USA, and the former Yugoslavia.

References:
1.  Kado, C.I. (1971). CMI/AAB Descr. Pl. Viruses No. 64, 4 pp.
2.  Tremaine, J.H. and Ronald, W.P. (1977). Can. J. Bot. 55: 2274.

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