Soybean Dwarf Virus (SbDV) - TAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V155
Source Antibody:  A rabbit polyclonal antibody for capture and and a monoclonal antibody as detection.
Test Format:  TAS ELISA                                   

Reactivity:  The test reacted with all SbDV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.800 - >2.600 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as soybean or other host plants. Background is very on all of  the negative control wells.

Application:  The test can be used to detect SbDV in infected host plant tissues.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V155-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V155-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V155-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V155-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V155-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V155-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V155-C1

Coating Antibody

500

115

2 lb (1 kg)

V155-C2

Coating Antibody

1000

205

2 lb (1 kg)

V155-C3

Coating Antibody

5000

725

2 lb (1 kg)

V155-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V155-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V155-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)

V155-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V155-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000  130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000  400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Soybean Dwarf Virus
Acronym:  SbDV
Synonyms:  subterranean clover red leaf luteovirus
Group/Genus:  Luteovirus

Vector:  Transmitted by a vector; an insect; Acyrthosiphon (Aulacorthum) solani (some isolates also by Acyrthosiphon pisum); Aphididae. Transmitted in a persistent manner. Virus retained when the vector moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; does not require a helper virus for vector transmission.
Transmission: Virus not transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed.
Main natural host plants and symptoms: Symptoms persist.

  • Glycine max, Trifolium pratense - puckering, rugosity and yellowing, stunting.
  • Pisum sativum - mild yellowing of older leaves.
  • Phaseolus vulgaris - yellowing of leaves and stunting.
  • Gomphrena globosa, Erodium moschatum, Trifolium subterraneum, Vicia sativa, Calandrinia caulescens - leaf reddening.
  • Beta vulgaris, Lupinus albus, Lupinus angustifolius, Medicago polymorpha - leaf yellowing.
  • Trifolium repens, T. pratense - no symptoms.
  • Vicia faba, Vicia articulata - leaf yellowing, rolling.

Diagnostically susceptible host species and symptoms: Astragalus sinicus - systemic leaf yellowing or reddening. Glycine max - systemic puckering, rugosity and yellowing of leaves, stunting. Trifolium incarnatum, T. subterraneum - systemic leaf reddening. Vicia faba - systemic chlorosis and yellowing.

Geographical distribution: Spreads in Australia, Japan, New Zealand, and the USA (in California).

References:
1. 
Johnstone, G.R., Duffus, J.E., Munro, D. and Ashby, J.W. (1982). Aust. J. agric. Res. 33: 697.
2.  Tamada, T. and Kojima, M. (1977). CMI/AAB Descr. Pl. Viruses No. 179, 4 pp.


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