Sweat Potato Feathery Mottle Virus (SPFMV) - DAS ELISA                Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V200
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all SPFMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.750 - 2.850 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as rice and other host plants. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect SPFMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)
V200-R1 ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)
V200-R2 ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)
V200-R3 ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V200-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V200-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V200-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)
V200-C1 Coating Antibody

500

125

2 lb (1 kg)
V200-C2 Coating Antibody

1000

235

2 lb (1 kg)
V200-C3 Coating Antibody

5000

805

2 lb (1 kg)
V200-D1 Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)
V200-D2 Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)
V200-D3 Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V200-P2 ELISA Control, Positive

18

15

1 lb (0.5 kg)
V200-N1 Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

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To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Sweat Potato Feathery Mottle Virus
Acronym:  SPFMV
Synonyms: sweet potato chlorotic leaf spot virus, sweet potato internal cork virus, sweet potato russet crack virus, sweet potato virus A (Hollings et al., 1976; Sheffield, 1957).
Group/Genus: Potyvirus

Vector:  Transmitted by a vector; an insect; Aphis gossypii, A. craccivora, Lipaphis erysimi, Myzus persicae; Aphididae. Transmitted in a non-persistent manner.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main host plants: Ipomoea spp.

Virus Infection: Symptoms disappear soon after infection (except for russet crack strain symptoms which persist on roots). vein clearing, vein feathering, chlorotic spots, in cv. Jersey also causes root necrosis if infected by russet crack strain.
Diagnostically susceptible host species and symptoms:
Ipomoea incarnata, I. nil - systemic vein clearing, vein banding, ringspots. I. setosa - vein clearing followed by remission.

Geographical distribution: Probably distributed worldwide. Found, but with no evidence of spread, in Louisiana, U.S.A.

 References:
1. 
Cohen, J., Salomon, R. and Loebenstein, G. (1988). Phytopathology 78: 809.
2.  Moyer, J.W. and Cali, B.B. (1985). J. gen. Virol. 66: 1185.


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