Sugarcane Mosaic Virus (SCMV) - DAS ELISA                                      Home    Products    Testing Services   Supplies

Catalog #:  V115
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test reacted equally with all isolates of SCMV tested. Reaction of the ELISA is very strong.  Optical Density at 405nm is in a range of 1.5 - 3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  This test system shows no cross-reaction with maize dwarf mosaic virus (MDMV) and johnsongrass mosaic virus (JGMV). There is also no cross-reaction found with other potyviruses tested, such as lettuce mosaic virus, papaya ringspot virus, potato virus A, potato virus Y, soybean mosaic virus, turnip mosaic virus, watermelon mosaic virus 2, and zucchini yellow mosaic virus. No cross reaction is found with healthy grasses, maize, sorghum, or sugarcane plant tissues in this test.  Background is very low on all of the negative control wells including buffer well..

Application:  The test can be used to detect SCMV in infected sugarcane and other cereal crops.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V115-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V115-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V115-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V115-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V115-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V115-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V115-C1

Coating Antibody

500

115

2 lb (1 kg)

V115-C2

Coating Antibody

1000

205

2 lb (1 kg)

V115-C3

Coating Antibody

5000

725

2 lb (1 kg)

V115-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V115-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V115-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)

V115-P1

Positive control

18

18

1 lb (0.5 kg)

V115-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase
500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase
1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase
5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Sugarcane Mosaic Virus
Acronym:  SCMV
Synonyms: Maize dwarf mosaic virus-B, abaca mosaic virus.
Group/Genus:  Potyviruses

Vector:  aphids
Transmission:  Mechanical transmission.  Seed transmission in maize.
Main host plants:  Maize, sugarcane, sorghum, abaca and grasses.

Viral Particle:  Sugarcane mosaic virus (SCMV), formerly called Maize dwarf mosaic virus-B (MDMV-B), is a potyvirus. The virus has a flexible filamentary particle about 750 nm long and contains a single strand of RNA.

Virus Infection:   SCMV is transmitted by several aphid species in a non-persistent manner and by mechanical inoculation. Seed transmission is found in maize, but not in sugarcane or sorghum. Based on host  pants, there are many strains identified for SCMV, such as USA A, B, D, E, F in sugarcane and rarely in Johnsongrass, Australian SCMV strains BC, Sabi in grasses and strain SC in sugarcane. Maize dwarf mosaic strain B infects maize but does not infect Johnsongrass or sugarcane. Abaca mosaic strain is found in abaca and maize. Typical symptom of SCMV infection is mosaic. SCMV occurs worldwide, but does not infect wheat, barley, rye or rice naturally.

Reference: 1. Ford, Tosic and Shukla, 1989, CMI/AAB Descr. Pl. Viruses, No. 342.
                    2. Shukla et al, 1989, Phytopathology 79: 223.
                    3. Shukla, Gough and Ward, 1987, Arch Virol. 96: 59.


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