Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Radish Mosaic Virus (RaMV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V198
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all RaMV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.750 - 2.850 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as rice and other host plants. Background is relative low on all of the negative control wells.
Application: The test can be used to detect RaMV in infected host plants.
Products:
|
Catalog Number |
Description | Test Wells | Price $US |
Ship
Weight (Estimated) |
| V198-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
240 |
2 lb (1 kg) |
| V198-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
415 |
3 lb (1.5 kg) |
| V198-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1660 |
12 lb (6 kg) |
| V198-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V198-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
355 |
2 lb (1 kg) |
| V198-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
565 |
3 lb (1.5 kg) |
| V198-C1 | Coating Antibody |
500 |
120 |
2 lb (1 kg) |
| V198-C2 | Coating Antibody |
1000 |
215 |
2 lb (1 kg) |
| V198-C3 | Coating Antibody |
5000 |
750 |
2 lb (1 kg) |
| V198-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
155 |
2 lb (1 kg) |
| V198-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
255 |
2 lb (1 kg) |
| V198-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
960 |
2 lb (1 kg) |
| V198-P2 | ELISA Control, Positive |
18 |
15 |
1 lb (0.5 kg) |
| V198-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test:
Instructions for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Radish Mosaic Virus
Acronym: RaMV
Synonyms: radish enation mosaic virus.
Group/Genus: comovirus
Vector: Transmitted by a vector; an insect; Phyllotreta spp.,
Epitrix hirtipennis and Diabrotica undecimpunctata; Coleoptera.
Transmission: Virus transmitted by mechanical inoculation; transmitted by
grafting; not transmitted by seed.
Main host plants: Brassica campestris, B. campestris ssp. napus,
B. oleracea, Raphanus sativus, Eruca sativa
Virus Infection: Symptoms persist. line and ring patterns, mosaic,
necrosis of veins and petioles, rosetting of leaves. mild mosaic, and leaf
deformation. variable; from symptomless to severe systemic chlorosis and
necrosis. mosaic, occasionally enations. severe crinkle, chlorosis, stunting,
some necrosis.
Diagnostically susceptible host species and symptoms: Brassica campestris
(tendergreen mustard) - necrotic local lesions; systemic mottling. B.
campestris (turnip) - chlorotic or necrotic local lesions; systemic mosaic
or line patterns. Chenopodium amaranticolor and other Chenopodium
spp. - local lesions; not systemic. Nicotiana tabacum cv. Turkish -
necrotic rings; not systemic.
References:
1. Mamula, D. and Juretic, N. (1985). Phyton (Houn, Austria)
25: 241.
2. Naqvi, Q.A., Khan, J.A. and Mahmood, K. (1986). J. Indian Bot.
Soc. 65: 136.