Radish Mosaic Virus (RaMV) - DAS ELISA                                         Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V198
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all RaMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.750 - 2.850 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as rice and other host plants. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect RaMV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)
V198-R1 ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)
V198-R2 ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)
V198-R3 ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V198-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V198-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V198-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)
V198-C1 Coating Antibody

500

125

2 lb (1 kg)
V198-C2 Coating Antibody

1000

235

2 lb (1 kg)
V198-C3 Coating Antibody

5000

805

2 lb (1 kg)
V198-D1 Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)
V198-D2 Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)
V198-D3 Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V198-P2 ELISA Control, Positive

18

15

1 lb (0.5 kg)
V198-N1 Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
   Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Radish Mosaic Virus
Acronym:  RaMV
Synonyms: radish enation mosaic virus.
Group/Genus: comovirus

Vector:  Transmitted by a vector; an insect; Phyllotreta spp., Epitrix hirtipennis and Diabrotica undecimpunctata; Coleoptera.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seed.
Main host plants: Brassica campestris, B. campestris ssp. napus, B. oleracea, Raphanus sativus, Eruca sativa

Virus Infection: Symptoms persist. line and ring patterns, mosaic, necrosis of veins and petioles, rosetting of leaves. mild mosaic, and leaf deformation. variable; from symptomless to severe systemic chlorosis and necrosis. mosaic, occasionally enations. severe crinkle, chlorosis, stunting, some necrosis.
Diagnostically susceptible host species and symptoms:
Brassica campestris (tendergreen mustard) - necrotic local lesions; systemic mottling. B. campestris (turnip) - chlorotic or necrotic local lesions; systemic mosaic or line patterns. Chenopodium amaranticolor and other Chenopodium spp. - local lesions; not systemic. Nicotiana tabacum cv. Turkish - necrotic rings; not systemic.

Geographical distribution: Spreads in Austria, Germany, Hungary, Italy, Japan, Morocco, the USA, the former USSR, and the former Yugoslavia.

 References:
1. 
Mamula, D. and Juretic, N. (1985). Phyton (Houn, Austria) 25: 241.
2.  Naqvi, Q.A., Khan, J.A. and Mahmood, K. (1986). J. Indian Bot. Soc. 65: 136.


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