Rice Yellow Mottle Virus (RYMV) - DAS ELISA                            Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V178
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all RYMV isolates tested. Reaction of the ELISA is very strong.  Optical Density at 405nm  is in arrange of 1.100 - >3.000 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as rice and other host plants. Background is very low on all of  the negative control wells.

Application:  The test can be used to detect RYMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V178-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V178-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V178-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V178-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V178-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V178-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V178-C1

Coating Antibody

500

125

2 lb (1 kg)

V178-C2

Coating Antibody

1000

235

2 lb (1 kg)

V178-C3

Coating Antibody

5000

805

2 lb (1 kg)

V178-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V178-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V178-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V178-P2

ELISA Control, Positive

18

18

1 lb (0.5 kg)

V178-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
   Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Rice Yellow Mottle Virus
Acronym:  RYMV
Synonyms:  
Group/Genus:  Sobemoviruses

Vector:  Transmitted by a vector; an insect; Sesselia pusilla, Chaetocnema pulla, Trichispa serica and Dicladispa viridicyanea; Coleoptera. Transmitted in a semi-persistent manner. Virus lost by the vector when it moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; does not require a helper virus for vector transmission.
Transmission: Virus transmitted by mechanical inoculation; not transmitted by seed.
Main host plants:  Oryza sativa, Oryza longistaminata - chlorosis and mottling.

Virus Infection:  Cause symptoms of yellow or orange leaves, stunting, reduced tillering and sterility, or chlorosis and mottling.  Symptoms persist.
Diagnostically susceptible host species and symptoms:
Oryza sativa - chlorotic dotting of leaf bases, elongating parallel to veins to produce mottling, stunting; Oryza barthii - yellow spots and vein streaking; Oryza punctata - systemic chlorotic dots.

Geographical distribution: Spreads in Burkina Faso, Cote d'Ivoire, Kenya, Liberia, Mali, Niger, Nigeria, Sierra Leone, and Tanzania (and, possibly, Zanzibar).

 References:
1. 
Bakker, W. (1975). CMI/AAB Descr. Pl. Viruses No. 149, 4pp.
2.  John, V.T., Thottappilly, G. and Awoderu, V.A. (1984). FAO Pl. Prot. Bull. 32: 86.


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