Rice Yellow Mottle Virus (RYMV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V178
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all RYMV isolates tested. Reaction of the ELISA is very strong. Optical Density at 405nm is in arrange of 1.100 - >3.000 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as rice and other host plants. Background is very low on all of the negative control wells.
Application: The test can be used to detect RYMV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V178-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
230 |
2 lb (1 kg) |
|
V178-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
395 |
3 lb (1.5 kg) |
|
V178-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1590 |
12 lb (6 kg) |
| V178-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V178-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
335 |
2 lb (1 kg) |
| V178-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
525 |
3 lb (1.5 kg) |
|
V178-C1 |
Coating Antibody |
500 |
115 |
2 lb (1 kg) |
|
V178-C2 |
Coating Antibody |
1000 |
205 |
2 lb (1 kg) |
|
V178-C3 |
Coating Antibody |
5000 |
725 |
2 lb (1 kg) |
|
V178-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
140 |
2 lb (1 kg) |
|
V178-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
225 |
2 lb (1 kg) |
|
V178-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
900 |
2 lb (1 kg) |
|
V178-P2 |
ELISA Control, Positive |
18 |
15 |
1 lb (0.5 kg) |
|
V178-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 80 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 105 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 335 | 6 lb (3.0 kg) |
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To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA In PDF Format
In PUB Format
To be familiar with other components: Buffer Sets -
PDF PUB; Controls -
PDF PUB
Information About the Virus
Name: Rice Yellow Mottle Virus
Acronym: RYMV
Synonyms:
Group/Genus: Sobemoviruses
Vector: Transmitted by a vector; an insect; Sesselia pusilla,
Chaetocnema pulla, Trichispa serica and Dicladispa viridicyanea;
Coleoptera. Transmitted in a semi-persistent manner. Virus lost by the vector
when it moults; does not multiply in the vector; not transmitted congenitally to
the progeny of the vector; does not require a helper virus for vector
transmission.
Transmission: Virus transmitted by mechanical inoculation; not
transmitted by seed.
Main host plants: Oryza sativa, Oryza longistaminata
- chlorosis and mottling.
Virus Infection: Cause symptoms of yellow or orange leaves,
stunting, reduced tillering and sterility, or chlorosis and mottling.
Symptoms persist.
Diagnostically susceptible host species and symptoms: Oryza sativa -
chlorotic dotting of leaf bases, elongating parallel to veins to produce
mottling, stunting; Oryza barthii - yellow spots and vein streaking;
Oryza punctata - systemic chlorotic dots.
Geographical distribution: Spreads in Burkina Faso, Cote d'Ivoire, Kenya, Liberia, Mali, Niger, Nigeria, Sierra Leone, and Tanzania (and, possibly, Zanzibar).
References:
1. Bakker, W. (1975). CMI/AAB Descr. Pl. Viruses No. 149, 4pp.
2. John, V.T., Thottappilly, G. and Awoderu, V.A. (1984). FAO
Pl. Prot. Bull. 32: 86.