Rice Ragged Stunt Virus (RRSV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V181
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all RRSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.700 - 2.600 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as rice and other host plants. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect RRSV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V181-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V181-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V181-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V181-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V181-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V181-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V181-C1

Coating Antibody

500

125

2 lb (1 kg)

V181-C2

Coating Antibody

1000

235

2 lb (1 kg)

V181-C3

Coating Antibody

5000

805

2 lb (1 kg)

V181-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V181-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V181-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V181-P2

ELISA Control, Positive

18

18

1 lb (0.5 kg)

V181-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
   Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Rice Ragged Stunt Virus
Acronym:  RRSV
Synonyms:  rice infectious gall virus
Group/Genus:  Oryzavirus

Vector:  Transmitted by a vector; an insect; Nilaparvata lugens; Delphacidae. Transmitted in a persistent manner. Virus retained when the vector moults; multiplies in the vector; not transmitted congenitally to the progeny of the vector; does not require a helper virus for vector transmission.
Transmission: Virus not transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main host plants:  Oryza latifolia, O. nivara, O. sativa.  

Virus Infection:  Cause symptoms of stunting, enations on veins of leaves and leaf sheaths, ragged leaves, flower suppression.  Symptoms persist.
Diagnostically susceptible host species and symptoms:
Oryza sativa cv. Taichung Native (TN-1) - pale spindle-shaped enations on abaxial surfaces of leaves and leaf sheaths, twisted and ragged leaves, dark green colour, suppressed flowering, infected plants remain green and appear grassy after healthy plants have senesced.

Geographical distribution: Spreads in Bangladesh, China, India, Indonesia, Japan, Malaysia, the Philippines, Sri Lanka, Taiwan, and Thailand.

 References:
1. 
Milne, R.G., Boccardo, G. and Ling, K.C. (1982). CMI/AAB Descr. Pl. Viruses No. 248, 5 pp.
2.  Yan, J., Kudo, H., Uyeda, I., Lee, S.Y. and Shikata, E. (1992). J. gen. Virol. 73: 785.


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