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PRODUCT ITEMS - PathogenID Test Kit
1. Components of PathogenID Text Kit
| Coated Plates |
96-well plates coated with specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) Empty plates for indirect ELISA |
| Detecting Conjugate | Alkaline Phosphatase, Specific antibody enzyme conjugate, or anti-species antibody enzyme conjugate. Enzyme conjugate is 1x solution and ready to use. |
| BluePhos Substrate | Part A and Part B, 1x. BluePhos substrate includes two parts of 1x solutions and is ready to use. Mix equal volumes of Part A and Part B in a container immediately before use. |
| Controls | Positive control and negative control, 18 test wells per battle. |
| Buffers | PBST Washing Buffer (powder), Tween-20 (for PBST and sample buffer), *SB1 General Sample buffer (powder), *SB4 grape sample buffer (powder), *SB5 blueberry sample buffer (powder) (*Only one of the three buffers is included in DAS and TAS ELISA) |
| Instruction | Specific test instructions for each of the three different PathogenID Formats. |
2. Contents of PathogenID
DAS ELISA, alkaline phosphatase conjugate, test instruction
| Item |
96 wells |
192 wells |
384 wells |
Storage |
| 96-well plates coated with antibody |
1 |
2 |
4 |
2-6oC |
| Detecting conjugate, alkaline phosphatase, 1x |
12 ml |
23 ml |
45 ml |
2-6oC |
| BluePhos substrate, Part A and Part B, 1x |
A: 6ml; B: 6ml |
A: 11ml; B: 11ml |
A: 22ml; B: 22ml |
2-6oC |
| ELISA control, positive, 1.8 ml/bottle |
1 |
1 |
2 |
2-6oC |
| ELISA control, negative, 1.8 ml/bottle |
1 |
1 |
2 |
2-6oC |
| PBST Washing buffer, powder |
14.4 g |
28.7 g |
57.3 g |
Room temperature |
| Tween-20, for PBST and sample buffer |
4.0 g |
7.0 g |
14.0 g |
Room temperature |
| SB1 General Sample buffer, powder* |
5.8 g |
11.6 g |
23.2 g |
Room temperature |
| SB4 grape sample buffer, powder* |
24.7 g |
49.4 g |
98.7 g |
Room temperature |
| SB5 blueberry sample buffer, powder* |
8.6 g |
17.3 g |
34.5 g |
Room temperature |
| Instruction |
1 |
1 |
1 |
|
**Only one of the three buffers is included
TAS ELISA, alkaline phosphatase conjugate, test instruction
| Item |
96 wells |
192 wells |
384 wells |
Storage |
| 96-well plates coated with antibody |
1 |
2 |
4 |
2-6oC |
| Detecting conjugate, alkaline phosphatase, Bottle A & Battle B, 1x |
A: 6ml; B: 6ml |
A: 12ml; B: 12ml |
A: 23ml; B: 23ml |
2-6oC |
| BluePhos substrate, Part A and Part B, 1x |
A: 6ml; B: 6ml |
A: 11ml; B: 11ml |
A: 22ml; B: 22ml |
2-6oC |
| ELISA control, positive, 1.8 ml/bottle |
1 |
1 |
2 |
2-6oC |
| ELISA control, negative, 1.8 ml/bottle |
1 |
1 |
2 |
2-6oC |
| PBST Washing buffer, powder |
14.4 g |
28.7 g |
57.3 g |
Room temperature |
| Tween-20, for PBST and sample buffer |
4.0 g |
7.0 g |
14.0 g |
Room temperature |
| SB1 General Sample buffer, powder* |
5.8 g |
11.6 g |
23.2 g |
Room temperature |
| SB4 grape sample buffer, powder* |
24.7 g |
49.4 g |
98.7 g |
Room temperature |
| SB5 blueberry sample buffer, powder* |
8.6 g |
17.3 g |
34.5 g |
Room temperature |
| Instruction |
1 |
1 |
1 |
|
Indirect ELISA, alkaline phosphatase conjugate, test instruction
| Item |
96 wells |
192 wells |
384 wells |
Storage |
| SB3 Indirect sample buffer, 10x |
25 ml |
50 ml |
100 ml |
2-6oC |
| Detecting conjugate, alkaline phosphatase, Bottle A & Battle B, 1x |
A: 6ml; B: 6ml |
A: 12ml; B: 12ml |
A: 23ml; B: 23ml |
2-6oC |
| BluePhos substrate, Part A and Part B, 1x |
A: 6ml; B: 6ml |
A: 11ml; B: 11ml |
A: 22ml; B: 22ml |
2-6oC |
| ELISA control, positive, 1.8 ml/bottle |
1 |
1 |
2 |
2-6oC |
| ELISA control, negative, 1.8 ml/bottle |
1 |
1 |
2 |
2-6oC |
| 96-well ELISA plates, empty |
1 |
2 |
4 |
Room temperature |
| PBST Washing buffer, powder |
14.4 g |
28.7 g |
57.3 g |
Room temperature |
| Tween-20, for PBST and sample buffer |
1.0 g |
2.0 g |
4.0 g |
Room temperature |
| Instruction |
1 |
1 |
1 |
|