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PRODUCT ITEMS - ELISA Kit

1. Components of ELISA Kit

Coating Antibody	Specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) for coating ELISA plate.
Detecting Conjugate	Specific antibody enzyme conjugate, or anti-species antibody enzyme conjugate.
ELISA Plate	Empty ELISA plate with high binding capacity. The plate should be used for our ELISA Reagents to achieve a maximum test results.
Controls	Positive control and negative control
Buffers	Coating buffer, sample buffer, enzyme conjugate buffer, washing buffer, substrate buffer and PNP tablets.
Instruction	Specific test instructions for each of the three different ELISA Formats.

2. Contents of ELISA Reagents

DAS ELISA, alkaline phosphatase conjugate

Item	500 wells	1000 wells	Storage
Coating antibody	^{0.25 ml}	^{0.50 ml}	^{4 oC}
^{Detecting conjugate, alkaline phosphatase}	^{0.25 ml}	^{0.50 ml}	^{4 oC}
^{96-well ELISA plates}	⁵	¹⁰	^{Room temperature}
Positive control	¹	²	^{4 oC}
Negative control	¹	²	^{4 oC}
Coating buffer, 10x	^{6 ml}	^{12 ml}	^{Room temperature}
Sample buffer powder	^{11.6 g}	^{23.2 g}	^{Room temperature}
Enzyme conjugate buffer, 10x	^{6 ml}	^{12 ml}	^{Room temperature}
PBST Washing buffer powder	^{28.7 g}	^57.23g	^{Room temperature}
Tween-20 for sample/washing buffers	^{7.0 g}	^{14.0 g}	^{Room temperature}
PNP substrate buffer, 5x	^{10 ml}	^{20 ml}	^{4 oC}
PNP tablet, 5mg each	^{10 tablets}	^{20 tablets}	^{-10-20 oC}
^Instruction	¹	¹

TAS ELISA, alkaline phosphatase conjugate

Item	500 wells	1000 wells	Storage
Coating antibody	0.25 ml	0.50 ml	4 ^oC
^{Detecting conjugate, alkaline phosphatase, ^{(Bottles A and B)}}	^{0.25 ml (A) 0.25 ml (B)}	^{0.50 ml (A) 0.50 ml (B)}	^{4 oC}
^{96-well ELISA plates}	⁵	¹⁰	^{Room temperature}
Positive control	¹	²	^{4 oC}
Negative control	¹	²	^{4 oC}
Coating buffer, 10x	^{6 ml}	^{12 ml}	^{Room temperature}
Sample buffer powder	^{11.6 g}	^{23.2 g}	^{Room temperature}
Enzyme conjugate buffer, 10x	^{6 ml}	^{12 ml}	^{Room temperature}
PBST Washing buffer powder	^{28.7 g}	^57.23g	^{Room temperature}
Tween-20 for sample/washing buffers	^{7.0 g}	^{14.0 g}	^{Room temperature}
PNP substrate buffer, 5x	^{10 ml}	^{20 ml}	^{4 oC}
PNP tablet, 5mg each	^{10 tablets}	^{20 tablets}	^{-10-20 oC}
^Instruction	¹	¹

Indirect ELISA, alkaline phosphatase conjugate

Item	500 wells	1000 wells	Storage
^{Detecting conjugate, alkaline phosphatase, ^{(Bottles A and B)}}	^{0.25 ml (A) 0.25 ml (B)}	^{0.50 ml (A) 0.50 ml (B)}	^{4 oC}
^{96-well ELISA plates}	⁵	¹⁰	^{Room temperature}
Positive control	¹	²	^{4 oC}
Negative control	¹	²	^{4 oC}
Sample buffer powder	^{11.6 g}	^{23.2 g}	^{Room temperature}
Enzyme conjugate buffer, 10x	^{6 ml}	^{12 ml}	^{Room temperature}
PBST Washing buffer powder	^{28.7 g}	^57.23g	^{Room temperature}
Tween-20 for washing buffers	^{7.0 g}	^{14.0 g}	^{Room temperature}
PNP substrate buffer, 5x	^{10 ml}	^{20 ml}	^{4 oC}
PNP tablet, 5mg each	^{10 tablets}	^{20 tablets}	^{-10-20 oC}
^Instruction	¹	¹

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