Pea Streak Virus (PeSV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V146
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all PeSV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.800 - >2.200 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as pea, bean, cowpea or other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect PeSV in infected pea, bean and other infected host plant tissues.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V146-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
230 |
2 lb (1 kg) |
|
V146-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
395 |
3 lb (1.5 kg) |
|
V146-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1590 |
12 lb (6 kg) |
| V146-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V146-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
335 |
2 lb (1 kg) |
| V146-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
525 |
3 lb (1.5 kg) |
|
V146-C1 |
Coating Antibody |
500 |
115 |
2 lb (1 kg) |
|
V146-C2 |
Coating Antibody |
1000 |
205 |
2 lb (1 kg) |
|
V146-C3 |
Coating Antibody |
5000 |
725 |
2 lb (1 kg) |
|
V146-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
140 |
2 lb (1 kg) |
|
V146-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
225 |
2 lb (1 kg) |
|
V146-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
900 |
2 lb (1 kg) |
|
V146-P2 |
ELISA control, positive |
18 |
12 |
1 lb (0.5 kg) |
|
V146-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Peas Streak Virus
Acronym: PeSV
Synonyms: alfalfa latent virus, Steinklee Virus, Wisconsin pea streak
virus, probably pea streak New Zealand virus (Bos, 1964; Chamberlain, 1954).
Group/Genus: Carlavirus
Vector: Transmitted by a vector; an insect; Acyrthosiphon pisum;
Aphididae. Transmitted in a non-persistent manner.
Transmission: Virus transmitted by mechanical inoculation; not
transmitted by contact between plants; not transmitted by seed; not transmitted
by pollen.
Main natural host plants: Medicago sativa, Pisum sativum.
Virus Infection: Symptoms by viral infection include
necrotic lesions or streaks. Symptoms persist, or disappear soon after infection
(in the glasshouse).
Diagnostically susceptible host species and symptoms: Pisum sativum
- inoculated leaves symptomless, systemic necrosis, vein banding, reddening.;
Vicia faba cv. Bell - necrotic local lesions, then systemic necrosis,
necrotic spots and streaks; Cassia occidentalis - necrotic or chlorotic
local lesions only.
Geographical distribution: Spreads in the Eurasian region; Canada and the USA.
References:
1. Hampton, R., Beczner, L., Hagedorn, D., Bos, L., Inouye, T.,
Barnett, O., Musil, M. and Meiners, J. (1978). Phytopathology 68:
989.
2. Veerisetty, V. (1979). CMI/AAB Descr. Pl. Viruses No.
211, 3 pp.