Pea Streak Virus (PeSV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V146
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all PeSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.800 - >2.200 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as pea, bean, cowpea or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect PeSV in infected pea, bean and other infected host plant tissues.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V146-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V146-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V146-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V146-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V146-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V146-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V146-C1

Coating Antibody

500

125

2 lb (1 kg)

V146-C2

Coating Antibody

1000

235

2 lb (1 kg)

V146-C3

Coating Antibody

5000

805

2 lb (1 kg)

V146-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V146-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V146-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V146-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V146-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Peas Streak Virus
Acronym:  PeSV
Synonyms:  alfalfa latent virus, Steinklee Virus, Wisconsin pea streak virus, probably pea streak New Zealand virus (Bos, 1964; Chamberlain, 1954).
Group/Genus:  Carlavirus

Vector:  Transmitted by a vector; an insect; Acyrthosiphon pisum; Aphididae. Transmitted in a non-persistent manner.
Transmission: Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main natural host plants: Medicago sativa, Pisum sativum.

Virus Infection:  Symptoms by viral infection include necrotic lesions or streaks. Symptoms persist, or disappear soon after infection (in the glasshouse).
Diagnostically susceptible host species and symptoms: Pisum sativum - inoculated leaves symptomless, systemic necrosis, vein banding, reddening.; Vicia faba cv. Bell - necrotic local lesions, then systemic necrosis, necrotic spots and streaks; Cassia occidentalis - necrotic or chlorotic local lesions only.

Geographical distribution: Spreads in the Eurasian region; Canada and the USA.

References:
1. 
Hampton, R., Beczner, L., Hagedorn, D., Bos, L., Inouye, T., Barnett, O., Musil, M. and Meiners, J. (1978). Phytopathology 68: 989.
2.  Veerisetty, V. (1979). CMI/AAB Descr. Pl. Viruses No. 211, 3 pp.


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