Peanut Mottle Virus (PeMoV) - DAS ELISA                                Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V162
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all PeMoV isolates tested. Reaction of the ELISA is strong.  Optical Density at 405_nm  is in arrange of 1.100 - >3.250 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as peanut or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect PeMoV in infected host plants.

Products: 

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To know more about the products:  Product Items
To learn how to perform the test: Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Peanut Mottle Virus
Acronym:  PeMoV
Synonyms:  groundnut mottle virus, peanut mild mosaic virus, peanut severe mosaic virus
Group/Genus:  Potyvirus

Vector:  Transmitted by a vector; an insect; Aphis craccivora, A. gossypii, Hyperomyzus lactucae, Myzus persicae, Rhopalosiphum padi; Aphididae. Transmitted in a non-persistent manner (A. craccivora can remain infective for 2 hours and M. persicae for 12 hours after acquisition (Paguio and Kuhn, 1974)).
Transmission: Virus transmitted by mechanical inoculation; transmitted by seed (0.02-2% in Arachis hypogaea; to 1% in Phaseolus vulgaris and to 0.008% in Vigna unguiculata (Demski et al., 1983), but not in Glycine max, Pisum sativum, Cassia obtusifolia).
Main natural host plants: Arachis hypogaea, Arachis pintoi, Cassia bicapsularis (east Africa) and C. leptocarpa, C. occidentalis, C. tora, Glycine max, Phaseolus vulgaris, Pisum sativum, Stylosanthes sp.

Virus Infection:  Symptoms include chlorotic ringspots in leave, mottling with necrosis and mosaic, leaf mottling and necrosis, mild leaf chlorosis and stunting. Symptoms persist.
Diagnostically susceptible host species and symptoms: Arachis hypogaea - necrotic local lesions, then systemic mottling, mosaic, necrosis; Cassia occidentalis - systemic mosaic; not infected by severe strain; Glycine max - systemic dark green mottling, crinkling, blistering and malformation; Phaseolus vulgaris - chlorotic local lesions; only some strains systemic; Nicotiana clevelandii - systemic mottling.

Geographical distribution: Spreads in the African region, the Eastern Asian region, and the South and Central American region; Australia (the north east), Colombia, India (possibly), Japan, Malaysia, the Philippines, Taiwan, the USA (in the south east).

References:
1.  Bock, K.R. and Kuhn, C.W. (1975). CMI/AAB Descr. Pl. Viruses No. 141, 4 pp.
2.  Morales, F.J., Castano, M., Velasco, A.C., Arroyave, J. and Zettler, F.W. (1991). Plant Dis. 75: 1090.

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