Peanut Mottle Virus (PeMoV) - DAS ELISA                                Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V162
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all PeMoV isolates tested. Reaction of the ELISA is strong.  Optical Density at 405nm  is in arrange of 1.100 - >3.250 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as peanut or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect PeMoV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V162-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V162-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V162-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V162-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V162-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V162-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V162-C1

Coating Antibody

500

125

2 lb (1 kg)

V162-C2

Coating Antibody

1000

235

2 lb (1 kg)

V162-C3

Coating Antibody

5000

805

2 lb (1 kg)

V162-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V162-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V162-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V162-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V162-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Peanut Mottle Virus
Acronym:  PeMoV
Synonyms:  groundnut mottle virus, peanut mild mosaic virus, peanut severe mosaic virus
Group/Genus:  Potyvirus

Vector:  Transmitted by a vector; an insect; Aphis craccivora, A. gossypii, Hyperomyzus lactucae, Myzus persicae, Rhopalosiphum padi; Aphididae. Transmitted in a non-persistent manner (A. craccivora can remain infective for 2 hours and M. persicae for 12 hours after acquisition (Paguio and Kuhn, 1974)).
Transmission: Virus transmitted by mechanical inoculation; transmitted by seed (0.02-2% in Arachis hypogaea; to 1% in Phaseolus vulgaris and to 0.008% in Vigna unguiculata (Demski et al., 1983), but not in Glycine max, Pisum sativum, Cassia obtusifolia).
Main natural host plants: Arachis hypogaea, Arachis pintoi, Cassia bicapsularis (east Africa) and C. leptocarpa, C. occidentalis, C. tora, Glycine max, Phaseolus vulgaris, Pisum sativum, Stylosanthes sp.

Virus Infection:  Symptoms include chlorotic ringspots in leave, mottling with necrosis and mosaic, leaf mottling and necrosis, mild leaf chlorosis and stunting. Symptoms persist.
Diagnostically susceptible host species and symptoms: Arachis hypogaea - necrotic local lesions, then systemic mottling, mosaic, necrosis; Cassia occidentalis - systemic mosaic; not infected by severe strain; Glycine max - systemic dark green mottling, crinkling, blistering and malformation; Phaseolus vulgaris - chlorotic local lesions; only some strains systemic; Nicotiana clevelandii - systemic mottling.

Geographical distribution: Spreads in the African region, the Eastern Asian region, and the South and Central American region; Australia (the north east), Colombia, India (possibly), Japan, Malaysia, the Philippines, Taiwan, the USA (in the south east).

References:
1. 
Bock, K.R. and Kuhn, C.W. (1975). CMI/AAB Descr. Pl. Viruses No. 141, 4 pp.
2.  Morales, F.J., Castano, M., Velasco, A.C., Arroyave, J. and Zettler, F.W. (1991). Plant Dis. 75: 1090.


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