Pectobacterium atrosepticum (Patr) - DAS ELISA                                Home    Products    Testing Services   Supplies

Catalog #:  B104
Source Antibody:  Rabbit polyclonal antibodies for both capture and detection.
Test Format:  DAS ELISA

Reactivity:  This ELISA reacted with all Pectobacterium atrosepticum (Patr) isolates tested.  Reaction of the ELISA is very strong.  Optical Density at 405_nm  is in a range of 1.700 - 3.000 depending  on the bacterial titers in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The bacterium can be consistently detected in infected plant tissues diluted at 1:2430.

Specificity:  This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis,  C. m. subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. carotovora, E. chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c. pv. diffenbachiae,  X. c. pv. pelargonii, X. c. pv. phaseoli, and X. c. pv. vesicatoria.  There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect Pectobacterium atrosepticum in infected host plants as well as in pure bacterial cultures.

Products: 

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA  In PDF Format  In PUB Format
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Bacterium

Name:  Pectobacterium atrosepticum
Acronym:  Patr
Synonyms: 
Group/Genus:  Pectobacterium

Host plants and Diseases:  Soft rot of potato. 
Symptoms:  Soft rot of tubers

Bacterial Transmission:  Infected plant residues, infested soil, agricultural equipments or activities.
Bacterial Infection:  Typical wound infection. Disease incidence increases when plants are injured by farming practices or wind.

Reference:

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