Pectobacterium atrosepticum -DAS-ELISA Home Products Testing Services Supplies
Catalog #: B104
Source Antibody: Rabbit polyclonal antibodies for both capture and
detection.
Test Format: DAS ELISA
Reactivity: This ELISA reacted with all Pectobacterium atrosepticum (Patr) isolates tested. Reaction of the ELISA is very strong. Optical Density at 405nm is in a range of 1.700 - 3.000 depending on the bacterial titers in the samples tested.
Sensitivity: Sensitivity of the ELISA is very high. The bacterium can be consistently detected in infected plant tissues diluted at 1:2430.
Specificity: This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, C. michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis, C. m. subsp. sepedonicus, C. m. subsp. tessellarius, Erwinia carotovora subsp. carotovora, E. chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c. pv. diffenbachiae, X. c. pv. pelargonii, X. c. pv. phaseoli, and X. c. pv. vesicatoria. There is no cross reaction with healthy plant tissues. Background is very low on all of the negative control wells.
Application: The test can be used to detect Pectobacterium atrosepticum in infected host plants as well as in pure bacterial cultures.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
B104-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
200 |
2 lb (1 kg) |
|
B104-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
350 |
3 lb (1.5 kg) |
|
B104-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1350 |
13 lb (6 kg) |
| V104-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V104-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
315 |
2 lb (1 kg) |
| V104-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
485 |
3 lb (1.5 kg) |
|
B104-C1 |
Coating Antibody |
500 |
100 |
2 lb (1 kg) |
|
B104-C2 |
Coating Antibody |
1000 |
175 |
2 lb (1 kg) |
|
B104-C3 |
Coating Antibody |
5000 |
640 |
2 lb (1 kg) |
|
B104-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
125 |
2 lb (1 kg) |
|
B104-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
200 |
2 lb (1 kg) |
|
B104-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
790 |
2 lb (1 kg) |
|
B104-P1 |
Positive control |
18 |
15 |
1 lb (0.5 kg) |
|
B104-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Bacterium
Name:
Pectobacterium atrosepticum
Acronym: Patr
Synonyms:
Group/Genus:
Pectobacterium
Host plants and Diseases: Soft rot of potato.
Symptoms: Soft rot of tubers
Bacterial Transmission: Infected plant residues, infested
soil, agricultural equipments or activities.
Bacterial Infection: Typical wound infection. Disease
incidence increases when plants are injured by farming practices or wind.
Reference:
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