Pepper Veinal Mottle Virus (PVMV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V177
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all PVMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.700 - 2.600 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as pepper and other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect PVMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V177-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V177-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V177-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V177-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V177-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V177-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V177-C1

Coating Antibody

500

125

2 lb (1 kg)

V177-C2

Coating Antibody

1000

235

2 lb (1 kg)

V177-C3

Coating Antibody

5000

805

2 lb (1 kg)

V177-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V177-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V177-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V177-P1

Positive control

18

18

1 lb (0.5 kg)

V177-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELIAS
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Pepper Veinal Mottle Virus
Acronym:  PVMV
Synonyms:  
Group/Genus:  Potyviruses

Vector:  Transmitted by a vector; an insect; Aphis gossypii, A. spiraecola, Myzus persicae, Toxoptera citricidus; Aphididae. Transmitted in a non-persistent manner.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants.
Main host plants:  Capsicum annuum, C. frutescens, Datura stramonium, Lycopersicon esculentum.

Virus Infection:  mottle, leaf shape malformation, mosaic, fruit malformation, with spine suppression, and necrotic or chlorotic spots and streaks. Symptoms persist.
Diagnostically susceptible host species and symptoms:
Chenopodium amaranticolor, C. foetidum - local chlorotic or necrotic ringspots; Capsicum annuum, C. frutescens - systemic mosaic, mottle and leaf shape malformation; Nicotiana clevelandii, N. megalosiphon, N. occidentalis, Physalis angulata, P. floridana - systemic mosaic. Nicotiana tabacum cv. Dutch A - local necrotic or chlorotic ringspots.

Geographical distribution: Spreads in Cote d'Ivoire, Ghana, Kenya, Nigeria, and South Africa.

 References:
1. 
Brunt, A.A. and Kenten, R.H. (1972). CMI/AAB Descr. Pl. Viruses No. 104, 4 pp.
2.  Ladipo, J.L. and Roberts, I.M. (1979). Pl. Dis. Reptr 63: 161.


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