Peanut Stunt Virus (PSV) - DAS ELISA                                                                Home   Products    Testing Services  

Catalog #:  V202
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all isolates tested. However, this test does not react with PSV-Western Strain. Reaction of the ELISA is strong.  Optical Density at 405_nm  is in arrange of 1.300 - >3.100 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as peanut, bean, pea, tobacco, or other host plants. Background is very low on all of  the negative control wells.

Application:  The test can be used to detect PSV in infected host plants.

Products: 

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To know more about the products:  Product Items
To learn how to perform the test: Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Peanut Stunt Virus
Acronym: PSV
Synonyms: robinia mosaic virus (Richter et al., 1979; Schmelzer, 1971; Zheng and Kung, 1984), black locust true mosaic virus, clover blotch virus (Richter et al., 1979).
Group/Genus:  Cucumovirus

Vector:  Transmitted by a vector; an insect; Aphis craccivora, A. spiraecola and Myzus persicae, but not Aphis gossypii; Aphididae. Transmitted in a non-persistent manner.
Transmission: Virus transmitted by mechanical inoculation; transmitted by seed (0.1% in Arachis hypogaea).
Main host plants: Robinia pseudoacacia, Trifolium repens, T. pratense, T. vesiculosum, T. incarnatum, T. subterraneum, Medicago sativa, Phaseolus spp., Arachis hypogaea, Vicia sp., Glycine max, Pisum sativum, Vigna angularis, Coronilla varia, Tephrosia sp., Nicotiana tabacum, Apium graveolens, Lupinus luteus.

Virus Infection Symptoms: mosaic and leaf malformation, chlorotic mottling,  stunting, pod malformation or leaf chlorosis. Symptoms persist.
Diagnostically susceptible host species and symptoms: Chenopodium amaranticolor, C. quinoa - chlorotic local lesions; systemic spotting; Spinacia oleracea, Tetragonia tetragonioides - systemic mottling; Glycine max - systemic chlorotic mottling, Phaseolus vulgaris - chlorotic or necrotic local lesions, systemic chlorotic mottling or mosaic; P. vulgaris cv. Bountiful - elongated mis-shapen trifoliate leaves; Pisum sativum - systemic chlorotic mottling, stunting; Vigna unguiculata - chlorotic local lesions; systemic vein clearing and severe epinasty, Datura stramonium - systemic chlorotic mottle with islands of green tissue; Nicotiana tabacum - light green and yellow rings, then systemic chlorotic areas; Tetragonia tetragonioides - systemic mottle.

Geographical distribution: Spreads in France, Japan, Korea D.P.R. (North), Korea Republic, Morocco, Poland, Spain, and the USA.

References:
1.  Karasawa, A., Nakaho, K., Kakutani, T., Minobe, Y. and Ehara, Y. (1991). Virology 185: 464.
2.  Mink, G.I. (1972). CMI/AAB Descr. Pl. Viruses No. 92, 4 pp.
3. Xu, Z., Barnett, O.W. and Gibson, P.B. (1986). Phytopathology 76: 390

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