Peanut Stunt Virus -Western Strain (PSV-WS)-DAS ELISA                Home   Products    Testing Services  

Catalog #:  V201
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all PSV-WS isolates tested. However, This test dose not reacted with the PSV typical strain. Reaction of the ELISA is strong.  Optical Density at 405nm  is in arrange of 1.000 - >2.600 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as peanut, bean, pea, tobacco, or other host plants. Background is very low on all of  the negative control wells.

Application:  The test can be used to detect PSV-WS in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)
V201-R1 ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)
V201-R2 ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)
V201-R3 ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V201-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V201-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V201-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)
V201-C1 Coating Antibody

500

125

2 lb (1 kg)
V201-C2 Coating Antibody

1000

235

2 lb (1 kg)
V201-C3 Coating Antibody

5000

805

2 lb (1 kg)
V201-D1 Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)
V201-D2 Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)
V201-D3 Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V201-P1 Positive control

18

15

1 lb (0.5 kg)
V201-N1 Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Peanut Stunt Virus-Western Strain
Acronym: PSV-WS
Synonyms:  robinia mosaic virus (Richter et al., 1979; Schmelzer, 1971; Zheng and Kung, 1984), black locust true mosaic virus, clover blotch virus (Richter et al., 1979).
Group/Genus:  Cucumovirus

Vector:  Transmitted by a vector; an insect; Aphis craccivora, A. spiraecola and Myzus persicae, but not Aphis gossypii; Aphididae. Transmitted in a non-persistent manner. .
Transmission: Virus transmitted by mechanical inoculation; transmitted by seed (0.1% in Arachis hypogaea).
Main host plants: Robinia pseudoacacia, Trifolium repens, T. pratense, T. vesiculosum, T. incarnatum, T. subterraneum, Medicago sativa, Phaseolus spp., Arachis hypogaea, Vicia sp., Glycine max, Pisum sativum, Vigna angularis, Coronilla varia, Tephrosia sp., Nicotiana tabacum, Apium graveolens, Lupinus luteus.

Virus Infection Symptoms: mosaic and leaf malformation, chlorotic mottling, chlorotic local lesions and mottling, stunting, pod malformation, or leaf chlorosis. Symptoms persist
Diagnostically susceptible host species and symptoms: Chenopodium amaranticolor, C. quinoa - chlorotic local lesions; systemic spotting; Spinacia oleracea, Tetragonia tetragonioides - systemic mottling; Glycine max - systemic chlorotic mottling; Phaseolus vulgaris - chlorotic or necrotic local lesions, systemic chlorotic mottling or mosaic; P. vulgaris cv. Bountiful - elongated mis-shapen trifoliate leaves; Pisum sativum - systemic chlorotic mottling, stunting; Vigna unguiculata - chlorotic local lesions; systemic vein clearing and severe epinasty; Datura stramonium - systemic chlorotic mottle with islands of green tissue; Nicotiana tabacum - light green and yellow rings, then systemic chlorotic areas; Tetragonia tetragonioides - systemic mottle.

Geographical distribution: Spreads in France, Japan, Korea D.P.R. (North), Korea Republic, Morocco, Poland, Spain, and the USA.

References:
1. 
Karasawa, A., Nakaho, K., Kakutani, T., Minobe, Y. and Ehara, Y. (1991). Virology 185: 464.
2.  Mink, G.I. (1972). CMI/AAB Descr. Pl. Viruses No. 92, 4 pp.
3. Xu, Z., Barnett, O.W. and Gibson, P.B. (1986). Phytopathology 76: 390


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