Plum Pox Virus (PPV) - TAS ELISA                                             Home    Products    Testing Services   Supplies

Catalog #:  V080
Source Antibody:  A rabbit polyclonal antibody for capture and a monoclonal antibody for detection.
Test Format:  TAS ELISA                                   

Reactivity:  This test system detects all isolates or strains of virus including D-strain and M-strain. Reaction of the ELISA is relatively strong.  Optical Density at 405_nm  is in a range of 1.000 - 2.800 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant leaf tissues diluted  at 1:810 - 1:2430.

Specificity:  This test does show some cross-reaction with some of other potyviruses. There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect PPV in infected host plants.

Products: 

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:  Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Plum Pox Virus
Acronym:  PPV
Synonyms:  Annulus pruni, Prunus virus 7,  sharka virus.
Group/Genus:  Potyviruses

Vector:  Aphids, in non-persistent manner.
Transmission:  mechanical
Main host plants:  Plum, peach, cherry, apricot, nectarine, almond, European plum, Janpanese plum

Viral Particle:  Filamentous particle, containing RNA genome.
Virus Infection: 

1.  CMI/AAB Descriptions of Plant Viruses, No.70.
2.  Adams, A. N. 1995. In Ogawa, J.M., ed. Compendium of Stone Fruit Diseases, APS Press, St. Paul. p. 69. (ISBN 0-89054-174-4)
3.  Candresse, T., Cambra, M., Dallot, S., Lanneau, M., Asensio, M., Gorris, M.T., Revers, F., Macquaire, A., Olmos, A., Boscia, D., Quiot, J.B., dunez, J. (1998) Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the D and M serotypes of Plum Pox Potyvirus. Phytopathology 88(3), 198-204.

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