Panicum Mosaic Virus (PMV) - DAS ELISA                          Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V170
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all PMV isolates tested. Reaction of the ELISA is very strong.  Optical Density at 405nm  is in arrange of 1.100 - >3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as panicum or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect PMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V170-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V170-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V170-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V170-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V170-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V170-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V170-C1

Coating Antibody

500

125

2 lb (1 kg)

V170-C2

Coating Antibody

1000

235

2 lb (1 kg)

V170-C3

Coating Antibody

5000

805

2 lb (1 kg)

V170-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V170--D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V170-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V170-P1

Positive control

18

20

1 lb (0.5 kg)

V170-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELIAS
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name: Panicum Mosaic Virus
Acronym:  PMV
Synonyms:  St. Augustine decline virus
Group/Genus:  Sobemovirus

Vector:
Transmission:
  Transmitted on cutting shears. Virus transmitted by mechanical inoculation; transmitted by seed (SAD), or not transmitted by seed (TS).
Main natural host plants: Panicum virgatum, Eremochloa ophiuroides, Muhlenbergia schreberi - .

Virus Infection:  Symptoms include faint chlorosis, chlorotic leaf spots, blotches and leaf chlorosis. Symptoms persist.
Diagnostically susceptible host species and symptoms: Zea mays inbred lines Ohio 28 and N28 - mild systemic mosaic (type strain only); Stenotaphrum secundatum - systemic chlorotic mottling (St. Augustine decline strain only); Avena sativa, Secale cereale, Hordeum vulgare, Triticum aestivum, Sorghum bicolor - chlorotic local lesions.

Geographical distribution: Spreads in Mexico and the USA.

References:
1. 
Haygood, R.A. and Barnett, O.W. (1992). Plant Dis. 76: 46.
2.  Niblett, C.L., Paulsen, A.Q. and Toler, R.W. (1977). CMI/AAB Descr. Pl. Viruses No. 177, 4 pp.


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