Prune Dwarf Virus (PDV) - TAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V154
Source Antibody:  A rabbit polyclonal antibody for capture and and a monoclonal antibody as detection.
Test Format:  TAS ELISA                                   

Reactivity:  The test reacted with all PDV isolates tested. Reaction of the ELISA is very strong.  Optical Density at 405nm  is in arrange of 0.900 - >3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted  at 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as peach, plum, apricot, or other host plants. Background is very low on all of  the negative control wells.

Application:  The test can be used to detect PDV in infected host plant tissues.

Products: 

Catalog Number

Description

Test Wells

Price
  $US

Ship Weight
(Estimated)

V154-R1

ELISA Reagents, Alkaline Phosphatase

500

210

2 lb (1 kg)

V154-R2

ELISA Reagents, Alkaline Phosphatase

1000

370

3 lb (1.5 kg)

V154-R3

ELISA Reagents, Alkaline Phosphatase

5000

1450

12 lb (6 kg)
V154-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V154-K1 ELISA Kit, Alkaline Phosphatase

500

325

2 lb (1 kg)
V154-K2 ELISA Kit, Alkaline Phosphatase

1000

505

3 lb (1.5 kg)
V154-K3 Pathogen ID Kit, Alkaline Phosphatase

96

185

1 lb (0.5 kg)
V154-K4 Pathogen ID Kit, Alkaline Phosphatase

192

245

2 lb (1 kg)
V154-K5 Pathogen ID Kit, Alkaline Phosphatase

384

435

3 lb (1.5 kg)

V154-C1

Coating Antibody

500

105

2 lb (1 kg)

V154-C2

Coating Antibody

1000

185

2 lb (1 kg)

V154-C3

Coating Antibody

5000

660

2 lb (1 kg)

V154-D1

Detecting conjugate, Alkaline Phosphatase

500

130

2 lb (1 kg)

V154-D2

Detecting conjugate, Alkaline Phosphatase

1000

205

2 lb (1 kg)

V154-D3

Detecting conjugate, Alkaline Phosphatase

5000

820

2 lb (1 kg)

V154-P2

ELISA control, positive

18

15

1 lb (0.5 kg)

V154-N1

Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500    90 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000     120 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000     350 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
   Instructions for DAS ELISA
To be familiar with other components:  Buffer Sets; Controls

Information About the Virus

Name:  Prune Dwarf Virus
Acronym:  PDV
Synonyms:  
Group/Genus:  Ilarvirus

Vector:  Transmitted by means not involving a vector.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; transmitted by seed (to 10% in Prunus cerasus); transmitted by pollen to the seed and transmitted by pollen to the pollinated plant.
Main natural host plants: VPrunus domestica, Prunus cerasus, Prunus persica.

Virus Infection:  Symptoms by viral infection include leathery, strap-like leaves, leaf yellowing and abscission, stunting. Symptoms persist.
Diagnostically susceptible host species and symptoms: Cucumis sativus - small chlorotic local lesions; systemic mosaic;  Sesbania exaltata - small dark local lesions in cotyledons; not systemic, Momordica balsamina - chlorotic local lesions; systemic mottle, Tithonia speciosa - prominent chlorotic lines and rings.

Geographical distribution: Probably distributed worldwide.

References:
1. 
Fulton, R.W. (1970). CMI/AAB Descr. Pl. Viruses No. 19, 3 pp.
2.  Halk, E.L. and Fulton, R.W. (1978). Virology 91: 434.


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