Potato Aucuba Mosaic Virus (PAMV) - DAS ELISA                          Home    Products    Testing Services   Supplies

Catalog #:  V0126
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all virus isolates tested.  Reaction of the ELISA is moderate.  Optical Density at 405_nm  is in arrange of 0.700 - 1.200 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.

Specificity:  There is no obvious cross reaction with healthy plant  tissues.  Background is low on the negative control wells incubated with healthy plant sample or sample buffer.

Application:  The test can be used to detect PAMV in infected host plants.

Products: 

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA  In PDF Format  In PUB Format
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Virus

Name:  Potato Aucuba Mosaic Virus
Acronym:  PAMV
Synonyms:  
Group/Genus:  Potexviruses

Vector:  
Transmission:  by a vector; an insect; Myzus persicae; Aphididae, and by mechanical transmission.
Main host plants:  Solanum tuberosum - chlorotic local lesions on older leaves, necrotic on younger, systemic (top) necrosis (Clinch, 1941). Stored tubers develop necrosis in the cortex and pith with irregular brown or sunken patches on the surface

Virus Infection:  cause chlorotic local lesions on older leaves, necrotic on younger, systemic (top) necrosis. Stored tubers develop necrosis in the cortex and pith with irregular brown or sunken patches on the surface.

References:
1.  Kassanis, B. and Govier, D.A. (1972). CMI/AAB Descr. Pl. Viruses No. 98, 4 pp.
2.  Harrison, B.D. and Jones, R.A.C. (1970). Ann. appl. Biol. 65: 393.

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