Okra Mosaic Virus (OkMV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V161
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all OkMV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.750 - >2.700 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as okra or other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect OkMV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V161-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
230 |
2 lb (1 kg) |
|
V161-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
395 |
3 lb (1.5 kg) |
|
V161-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1590 |
12 lb (6 kg) |
| V161-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V161-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
335 |
2 lb (1 kg) |
| V161-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
525 |
3 lb (1.5 kg) |
|
V161-C1 |
Coating Antibody |
500 |
115 |
2 lb (1 kg) |
|
V161-C2 |
Coating Antibody |
1000 |
205 |
2 lb (1 kg) |
|
V161-C3 |
Coating Antibody |
5000 |
725 |
2 lb (1 kg) |
|
V161-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
140 |
2 lb (1 kg) |
|
V161-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
225 |
2 lb (1 kg) |
|
V161-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
900 |
2 lb (1 kg) |
|
V161-P2 |
ELISA control, positive |
18 |
15 |
1 lb (0.5 kg) |
|
V161-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions for DAS
ELIAS
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Okra Mosaic Virus
Acronym: OkMV
Synonyms: virus de la mosaique du Gombo
Group/Genus: Tymovirus
Vector: Transmitted by a vector; an insect; Podagrica decolorata
in C“te d'Ivoire (Givord and den Boer, 1980); Podagrica uniforma, P.
sjostedti in Nigeria (Lana and Taylor, 1976; Atiri, 1984). Possibly
Bemisia tabaci is a vector in Nigeria (Lana and Taylor, 1976), but needs
confirmation; Coleoptera. Not transmitted by Aphis gossypii, Chrysolagria
cuprina, Lagria villosa, Medythia quaterna, Ootheca mutabilis, Nisotra dilecta.
Transmitted in a non-persistent manner.
Transmission: Virus transmitted by mechanical inoculation; transmitted by
grafting; not transmitted by seed.
Main natural host plants: Abelmoschus esculentus, Hibiscus
rosa-sinensis, Abutilon hirtum, Borreria intricans,
Corchorus olitorius, Hibiscus sabdariffa, Malvastrum coromandelianum,
Sida acuta, S. rhomboidea, Physalis angulata, Sida linifolia -
mosaic and vein banding, Urena lobata.
Virus Infection: Symptoms persist (in all other families except
Malvaceae). Symptoms mosaic, vein chlorosis and banding in Malvaceae. Other
families show interveinal chlorosis, vein chlorosis and vein banding. In all
families plants are often stunted.
Diagnostically susceptible host species and symptoms: Chenopodium
amaranticolor - systemic chlorotic spotting and line patterns; Cucumis
sativus - large chlorotic local lesions, then systemic vein chlorosis;
Arachis hypogaea - dark and light green mosaic; Vigna unguiculata -
fine systemic vein chlorosis and mosaic; Nicotiana clevelandii - systemic
dark green and whitish mosaic, dwarfing.
Geographical distribution: Spreads in Cote d'Ivoire and Nigeria.
References:
1. Atiri, G.I., Ivbijaro, M.F. and Oladele, A.D. (1991). Trop.
Agric., Trin. 68: 178.
2. Givord, L. and Koenig, R. (1974). CMI/AAB Descr. Pl. Viruses
No. 128, 4 pp.