Maize White Line Mosaic Virus (MWLMV) - DAS ELISA             Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V158
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all MWLMV isolates tested. Reaction of the ELISA is very strong.  Optical Density at 405nm  is in arrange of 1.100 - >3.000 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted at 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as corn and wheat. Background is very low on all of  the negative control wells.

Application:  The test can be used to detect MWLMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells

Price
  $US

Ship Weight
(Estimated)

V158-R1

ELISA Reagents, Alkaline Phosphatase

500

210

2 lb (1 kg)

V158-R2

ELISA Reagents, Alkaline Phosphatase

1000

370

3 lb (1.5 kg)

V158-R3

ELISA Reagents, Alkaline Phosphatase

5000

1450

12 lb (6 kg)
V158-R4 ELISA Reagents, Alkaline Phosphatase

100

75

1 lb (0.5 kg)
V158-K1 ELISA Kit, Alkaline Phosphatase

500

315

2 lb (1 kg)
V158-K2 ELISA Kit, Alkaline Phosphatase

1000

495

3 lb (1.5 kg)

V158-C1

Coating Antibody

500

105

2 lb (1 kg)

V158-C2

Coating Antibody

1000

185

2 lb (1 kg)

V158-C3

Coating Antibody

5000

660

2 lb (1 kg)

V158-D1

Detecting conjugate, Alkaline Phosphatase

500

130

2 lb (1 kg)

V158-D2

Detecting conjugate, Alkaline Phosphatase

1000

205

2 lb (1 kg)

V158-D3

Detecting conjugate, Alkaline Phosphatase

5000

820

2 lb (1 kg)

V158-P1

Positive control

18

15

1 lb (0.5 kg)

V158-N1

Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500    80 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000     105 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000     335 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA  In PDF Format  In PUB Format
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Virus

Name:  Maize White Line Mosaic Virus
Acronym:  MWLMV
Synonyms:  maize dwarf ringspot virus, possibly maize white line virus
Group/Genus: 

Vector: 
Transmission: Virus not transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main host plants:  Zea mays, Digitaria sanguinalis, Panicum dichotomiflorum, Setaria faberi, S. viridis

Virus Infection:  Cause symptoms of mottle, mosaic and characteristic chlorotic white lines, small ears with few or no kernels. Symptoms persist.
Diagnostically susceptible host species and symptoms: Zea mays - mottle, mosaic, discrete chlorotic white lines, stunting.

Geographical distribution: Spreads in France, Italy, and the USA.

References:
1. 
Gingery, R.E. and Louie, R. (1984). Phytopathology 74: 808.
2.  Louie, R., Gordon, D.T., Madden, L.V. and Knoke, J.K. (1983). Plant Dis. 67: 371.


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