Maize Streak Virus (MSV) - DAS ELISA                                      Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V210
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all MSV isolates tested. Reaction of the ELISA is strong.  Optical Density at 405nm  is in arrange of 0.700 - >2.950 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as corn and wheat. Background is very low on all of  the negative control wells.

Application:  The test can be used to detect MSV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V210-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V210-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V210-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V210-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V210-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V210-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V210-C1

Coating Antibody

500

115

2 lb (1 kg)

V210-C2

Coating Antibody

1000

205

2 lb (1 kg)

V210-C3

Coating Antibody

5000

725

2 lb (1 kg)

V210-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V210-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V210-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)

V210-P1

Positive control

18

18

1 lb (0.5 kg)

V210-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Maize StreakMosaic Virus
Acronym:  MSV
Synonyms:  bajra streak virus (Seth et al., 1972a & 1972b), cereal African streak virus, maize streak A virus.
Group/Genus:  Monogeminivirus

Vector:  Transmitted by a vector; an insect; Cicadulina mbila, C. arachidis, C. bipunctella, C. triangula, C. bimaculata, C. similis, C. latens, C. ghaurii, C. parazeae; Cicadellidae. Transmitted in a persistent manner. Virus retained when the vector moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector.
Transmission: Virus not transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main host plants:  Avena sativa, Hordeum vulgare, Secale cereale, Sorghum bicolor, Triticum aestivum, Zea mays, Saccharum spp., Panicum maximum, Setaria verticillata, Digitaria abyssinica, Digitaria velutina, Cenchrus echinatus, Brachiaria reptens, Coix lacryma-jobi, Chloris gayana, Pennisetum purpureum, Sorghum sudanense, Eleusine africana, Eleusine indica, Rottboellia exaltata, Setaria homonyma, Setaria pallidifusca, Urochloa panicoides, Urochloa trichopus

Virus Infection:  Causes symptoms of chlorotic or white streaking or lesions. Symptoms persist.
Diagnostically susceptible host species and symptoms: Zea mays - systemic chlorotic streaking.

Geographical distribution: Spreads in the African region; India, Madagascar, Reunion, and Yemen.

References:
1. 
Bock, K. (1974). CMI/AAB Descr. Pl. Viruses No. 133, 4 pp.
2.  Boulton, M.I., King, D.I., Donson, J. and Davies, J.W. (1991). Virology 183: 114.
3. Howarth, A.J. and Vandemark, G.J. (1989). J. gen. Virol. 70: 2717.


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