Maize Chlorotic Mottle Virus (MCMV) - DAS ELISA                       Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V219
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all MCMV isolates tested. Reaction of the ELISA is strong.  Optical Density at 405nm  is in arrange of 1.100 - 2.950 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as corn and wheat. Background is low on all of  the negative control wells.

Application:  The test can be used to detect MCMV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V219-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V219-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V219-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V219-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V219-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V219-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V219-C1

Coating Antibody

500

115

2 lb (1 kg)

V219-C2

Coating Antibody

1000

205

2 lb (1 kg)

V219-C3

Coating Antibody

5000

725

2 lb (1 kg)

V219-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V219-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V219-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)

V219-P1

Positive control

18

20

1 lb (0.5 kg)

V219-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA 
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Maize Chlorotic Mottle Virus
Acronym:  MCMV
Synonyms:
Group/Genus:  Machlomovirus

Vector:  Transmitted by a vector; an insect; Diabrotica spp. and thrips; Coleoptera, or Thysanoptera. Transmitted in a non-persistent manner. .
Transmission: Virus transmitted by mechanical inoculation; transmitted by seed (detected in 17 of 42000 seedlings from 25 seed lots).
Main host plants:  Zea mays

Virus Infection:  Cause systemic chlorotic leaf mottling; in mixtures with any of several potyviruses also causes necrosis, stunting and death.
Diagnostically susceptible host species and symptoms: Zea mays - systemic chlorotic leaf mottling; Triticum aestivum - systemic chlorotic leaf spotting and mottling.

Geographical distribution: Spreads in Argentina, Mexico, Peru, and the USA (Kansas, Nebraska and Hawaii).

References:
1. 
Goldberg, K.B. and Brakke, M.K. (1987). Phytopath. 77: 162.
2.  Lommel, S.A., Kendall, T.L., Siu, N.F. and Nutter, R.C. (1991). Phytopathology 81: 819.


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