Maize Chlorotic Dwarf Virus (MCDV) - DAS ELISA                 Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V160
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all MCDV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.600 - >2.600 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as corn or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect MCDV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V160-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V160-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V160-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V160-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V160-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V160-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V160-C1

Coating Antibody

500

125

2 lb (1 kg)

V160-C2

Coating Antibody

1000

235

2 lb (1 kg)

V160-C3

Coating Antibody

5000

805

2 lb (1 kg)

V160-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V160-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V160-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V160-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V160-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
   Instructions for DAS ELISA 
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Maize Chlorotic Dwarf Virus
Acronym:  MCDV
Synonyms:  Ohio corn stunt agent
Group/Genus:  Waikavirus

Vector:  Transmitted by a vector; an insect; Graminella nigrifrons most important, but also Graminella sonora and Exitianus exitiosus; Cicadellidae. Transmitted in a semi-persistent manner. Virus lost by the vector when it moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector.
Transmission: Virus is not transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main natural host plants: Zea mays, Sorghum halepense.

Virus Infection:  Symptoms include stunting, reddening and yellowing, tertiary vein banding, mild vein clearing. Symptoms persist.
Diagnostically susceptible host species and symptoms: Digitaria sanguinalis, Panicum miliaceum, Pennisetum glaucum, Sorghum vulgare var. sudanense - symptoms mild or absent;  Setaria faberi, S. lutescens; Sorghum bicolor, S. halepense - mild vein clearing; Triticum aestivum - symptomless; Zea mays - stunting, yellowing and reddening, tertiary vein banding.

Geographical distribution: Spreads in the USA (the south).

References:
1. 
Gingery, R.E., Bradfute, D.E., Gordon, D.T. and Nault, L.R. (1978). CMI/AAB Descr. Pl. Viruses No. 194, 4 pp.
2.   Maroon, C.M., Gordon, D.T. and Ginger, R.E. (1989). Phytopathology 79: 1157


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