Lettuce Mosaic Virus (LMV) - TAS ELISA                                         Home    Products    Testing Services   Supplies

Catalog #:  V174
Source Antibody:  A rabbit polyclonal antibody for capture and and a monoclonal antibody as detection.
Test Format:  TAS ELISA                                   

Reactivity:  This test system detects all isolates or strains of virus.  Reaction of the ELISA is very strong.  Optical Density at 405nm  is in a range of 1.200 - >3.000 for leaf samples and 0.400 - 1.400 for seed samples, depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected tissues diluted at 1:2430 for leaf samples and 1:30-1:90 for seed samples. This ELISA can be used to detection LMV in lettuce seeds.

Specificity:  There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells, include lettuce seeds..

Application:  The test can be used to detect LMV in infected host plant tissues such as leaves and seeds..

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V174-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V174-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V174-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V174-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V174-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V174-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V174-C1

Coating Antibody

500

125

2 lb (1 kg)

V174-C2

Coating Antibody

1000

235

2 lb (1 kg)

V174-C3

Coating Antibody

5000

805

2 lb (1 kg)

V174-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V174-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V174-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V174-P1

Positive control

18

18

1 lb (0.5 kg)

V174-N1

Negative control

18

18

1 lb (0.5 kg)
M174-1 Buffers for DAS/TAS, alkaline phosphatase 500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Lettuce Mosaic Virus
Acronym:  LMV
Synonyms:  Lactuca virus 1, Marmor lactucae.
Group/Genus:  Potyviruses

Vector:  Transmitted by a vector; an insect; Aphis gossypii, Macrosiphum euphorbiae, M. gei, Myzus persicae; Aphididae. Transmitted in a non-persistent manner.
Transmission:
  Virus transmitted by mechanical inoculation; transmitted by seed (3-10%); transmitted by pollen to the seed.
Main host plants:  Lettuce

Viral Particle:  Filamentous particle, containing RNA genome.
Virus Infection:   Lettuce mosaic virus (LMV) causes leaf mosaic, vein band and plant stunting in infected lettuce.  LMV is transmitted by aphids in non-persistent manner.  Rates of seed transmission is about 10 percent.

Geographical distribution: Probably distributed worldwide (wherever Lactuca sativa is grown).

1.  Pink, D.A.C., Kostova, D. and Walkey, D.G.A. (1992). Plant Path. 41: 5.
2. 
CMI/AAB Descriptions of Plant Viruses, No. 9.


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