Johnsongrass Mosaic Virus (JGMV) - DAS ELISA                      Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V113
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test reacted equally with all isolates of JGMV tested. Reaction of the ELISA is strong.  Optical Density at 405nm is in a range of 0.900 - 2.600 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted at 1:810.

Specificity:  This test system shows no cross-reaction with maize dwarf mosaic virus (MDMV) and sugarcane mosaic virus (SCMV). There is also no cross-reaction found with other potyviruses tested, such as lettuce mosaic virus, papaya ringspot virus, potato virus A, potato virus Y, soybean mosaic virus, turnip mosaic virus, watermelon mosaic virus 2, and zucchini yellow mosaic virus. No cross reaction is found with healthy grasses, maize, sorghum, or sugarcane plant tissues in this test.  Background is very low on all of the negative control wells including buffer well..

Application:  The test can be used to detect JGMV in infected maize and other cereal crops.

Products: 

Catalog Number

Description

Test Wells

Price $USD Ship Weight
(Estimated)

V113-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V113-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V113-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V113-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V113-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V113-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V113-C1

Coating Antibody

500

125

2 lb (1 kg)

V113-C2

Coating Antibody

1000

235

2 lb (1 kg)

V113-C3

Coating Antibody

5000

805

2 lb (1 kg)

V113-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V113-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V113-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V113-P1

Positive control

18

15

1 lb (0.5 kg)

V113-N1

Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Johnsongrass Mosaic Virus
Acronym:  JGMV
Synonyms: Maize dwarf mosaic virus-O
Group/Genus:  Potyviruses

Vector:  aphids
Transmission:  Mechanical transmission.  No seed transmission.
Main host plants:  Maize, sorghum and grasses.

Viral Particle:  Johnsongrass mosaic virus (JGMV) is a potyvirus, with flexible filamentary particle about 750 nm long and containing a single strand of RNA.
Virus Infection: 
The virus is transmitted by several aphid species in a non-persistent manner and by mechanical inoculation.  Seed transmission is not found for this virus.  JGMV infects maize, sorghum and grasses with mosaic symptom.  JGMV is found in Australia and the USA, with a type strain (Australian Johnsongrass), a Krish strain and a Texas strain.

Reference: 1. Ford, Tosic and Shukla, 1989, CMI/AAB Descr. Pl. Viruses, No. 340.
                    2. McDaniel and Gordon, 1985, Pl. Dis. 69: 62.
                    3. Tosic, Ford, Shukla and Jilka, 1990, Pl. Dis. 74: 549.


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