Catalog #: V113
Reactivity: This test reacted equally with all isolates of JGMV tested. Reaction of the ELISA is strong. Optical Density at 405nm is in a range of 0.900 - 2.600 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: This test system shows no cross-reaction with maize dwarf mosaic virus (MDMV) and sugarcane mosaic virus (SCMV). There is also no cross-reaction found with other potyviruses tested, such as lettuce mosaic virus, papaya ringspot virus, potato virus A, potato virus Y, soybean mosaic virus, turnip mosaic virus, watermelon mosaic virus 2, and zucchini yellow mosaic virus. No cross reaction is found with healthy grasses, maize, sorghum, or sugarcane plant tissues in this test. Background is very low on all of the negative control wells including buffer well..
Application: The test can be used to detect JGMV in infected maize and other cereal crops.
It is easy and convenient To Place
Information About the Virus
Name: Johnsongrass Mosaic Virus
Viral Particle: Johnsongrass mosaic virus (JGMV) is a
potyvirus, with flexible filamentary particle about 750 nm long and containing a
single strand of RNA.
Reference: 1. Ford, Tosic and Shukla, 1989, CMI/AAB Descr. Pl.
Viruses, No. 340.
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